Stress-endureing cells from oral tissues for bone engineering

用于骨工程的口腔组织耐压细胞

• 批准号: 24792145
• 负责人: TAJIMA Nobutaka
• 金额: $ 2.66万
• 依托单位: Nagasaki University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24792145/
• 关键词: 骨再生; ストレス耐性; 間葉系幹細胞; 骨再生医療; 多能性幹細胞

Human periodontal ligament cells were exposed to severe hypoxic (O2<0.1%) condition in culture to isolate the stress-enduring cells for bone engineering. Cells survived under this condition for 12 hours showed the high expression of pluripotent stem cell markers as well as mesenchymal stem cell markers, and also displayed the highest pluripotency in vitro. In particular, these surviving cells after re-oxygenation could form abundant calcified-nodules in osteogenic medium. Furthermore, a complex with these stress-enduring cells and fibrin-gel showed the remarkable osteoinducibility in vivo.

采用低氧（O2<0.1%）培养人牙周膜细胞，以分离耐应力细胞用于骨工程。在此条件下存活12小时的细胞显示出多能干细胞标志物以及间充质干细胞标志物的高表达，并且还显示出最高的体外多能性。特别是，这些存活的细胞在复氧后可以在成骨培养基中形成丰富的钙化结节。此外，这些耐应力细胞与纤维蛋白凝胶的复合物在体内显示出显著的骨诱导作用。

项目成果

Evaluation of sinus floor augmentation with simultaneous implant placement using autologous fibrin clot in concentrated growth factors (CGF) as sole grafting material.

使用浓缩生长因子（CGF）中的自体纤维蛋白凝块作为唯一的移植材料，对窦底增强术和同步种植体植入进行评估。

• 发表时间: 2012
• 作者: Tajima N;Ohba S;Sawase T;Asahina I
• 通讯作者: Asahina I

Evaluation of sinus floor augmentation with simultaneous implant placement using autologous figrin clot in concentrated growth factors (CGF) as sole grafting material

使用浓缩生长因子 (CGF) 中的自体 Figrin 凝块作为唯一的移植材料，评估上颌窦底增强术和同步种植体植入

• 发表时间: 2012
• 作者: Tajima N;Ohba S;Sawase T;Asahina I
• 通讯作者: Asahina I

Evaluation of Sinus Floor Augmentation with Simultaneous Implant Placement Using Platelet-Rich Fibrin as Sole Grafting Material

• DOI: 10.11607/jomi.2613
• 发表时间: 2013-01-01
• 期刊: INTERNATIONAL JOURNAL OF ORAL & MAXILLOFACIAL IMPLANTS
• 影响因子: 2
• 作者: Tajima, Nobutaka;Ohba, Seigo;Asahina, Izumi
• 通讯作者: Asahina, Izumi