Effects of local blood flow improving agent on the periodontal tissue regeneration

MAchR家族均在mRNA水平表达于MPDL22。在长期成骨培养过程中，卡普兰铬处理可增强成骨相关基因Runx2、Osterix、ALP、I型胶原、骨钙素、骨桥蛋白、骨联素的表达和碱性磷酸酶的活性。此外，氯化卡普因还促进了MPDL22细胞钙化结节的形成。此外，还证实了卡普兰铬促进人脐静脉内皮细胞在基质上的成管作用，提示卡普兰铬盐在牙周疾病中的应用可以促进牙周组织再生。

All of the mAchR family were expressed in MPDL22 at the mRNA level. Carpronium chrolide treatment enhanced the ALP activity and expression of ossification related genes, such as Runx2, Osterix, ALP, Collagen type I, Osteocalcin, Osteopontin, and Osteonectin, in the period of long term ossification culture. Furthermore, Carpronium chloride promoted calcified nodule formation of MPDL22 cells. In addition, it was confirmed that carpronium chrolide accelerated the tube formation of HUVECs on matrigel.These data suggest that application of carpronium chrolide on periodontal disease could stimulate periodontal tissue regeneration.