Effects of local blood flow improving agent on the periodontal tissue regeneration

所有mAchR家族在mRNA水平上在MPDL 22中表达。在长期的成骨培养过程中，卡泼罗尼处理增强了ALP活性和成骨相关基因如Runx 2、Osterix、ALP、I型胶原、骨钙素、骨桥蛋白和骨连接素的表达。此外，卡品氯铵促进MPDL 22细胞的钙化结节形成。此外，还证实了铬化卡普罗宁可促进HUVECs在Matrigel上的成管，提示应用铬化卡普罗宁治疗牙周病可促进牙周组织再生。

All of the mAchR family were expressed in MPDL22 at the mRNA level. Carpronium chrolide treatment enhanced the ALP activity and expression of ossification related genes, such as Runx2, Osterix, ALP, Collagen type I, Osteocalcin, Osteopontin, and Osteonectin, in the period of long term ossification culture. Furthermore, Carpronium chloride promoted calcified nodule formation of MPDL22 cells. In addition, it was confirmed that carpronium chrolide accelerated the tube formation of HUVECs on matrigel.These data suggest that application of carpronium chrolide on periodontal disease could stimulate periodontal tissue regeneration.