Periodontitis and antibodies to Porphyromonas gignivalis: The application of the enzyme-labeled antigen method

组织学上，牙周炎的牙龈组织中有致密的浆细胞浸润。 然而，由免疫细胞分泌的抗体识别的抗原仍然未知。 牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）是牙周炎的主要致病菌。因此，这是可能的，这些免疫细胞产生抗体的抗原的Pg。在本研究中，我们试图证明Pg特异性抗体产生细胞在牙龈组织的牙周炎与AlphaScreen方法和酶标记抗原的方法。结果，在患病牙龈中检测到产生特异于Pg抗原的抗体的浆细胞。这一发现表明，炎症反应Pg参与牙周炎。

Histologically, the gingival tissue in periodontitis shows dense infiltration of plasma cells. However, antigens recognized by antibodies secreted from the immunocytes remain unknown. Porphyromonas gingivalis (Pg) is the major pathogen of periodontitis. Therefore, it is possible that these immunocytes produce antibodies to antigens of Pg. In the present research, we attempted to demonstrate the Pg-specific antibody producing cells in the gingival tissues of periodontitis with the AlphaScreen method and the enzyme-labeled antigen method. As a result, the plasma cells producing antibodies specific to Pg antigens were detected in the diseased gingiva. This finding suggests inflammatory reactions to Pg is involved in periodontitis.