AlphaScreen Technology for High Throughput Screenin(RMI)

用于高通量筛选的 AlphaScreen 技术 (RMI)

• 批准号: 7058035
• 负责人: HAIAN FU
• 金额: $ 0.46万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-15 至 2006-09-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7058035
• 关键词: binding proteins; biological signal transduction; biotechnology; cell communication molecule; combinatorial chemistry; drug screening /evaluation; high throughput technology; inhibitor /antagonist; phosphoserine; protein protein interaction; small molecule; technology /technique development

DESCRIPTION (provided by applicant): Through phosphorylation-dependent binding to target proteins, 14-3-3 proteins regulate a wide range of physiological processes, including cell proliferation, cell cycle progression, suppression of apoptotic program, and neuronal functions. In order to generate research tools for study the functions of 14-3-3 in various biological systems and potentially to develop 14-3-3-targeted therapeutic strategies, we have developed an AlphaScreen technology based high throughput assay for isolation of small molecules that can disrupt the 14-3-3/client protein interactions. We have optimized and miniaturized the assay for adaptation in a 384-well plate format for robotic operations. The goal of this proposal is to utilize this assay in the Molecular Libraries Screening Centers Network for isolation of small molecule compounds that can interfere with the interaction of 14-3-3 with its client proteins.

描述（由申请人提供）：14-3-3蛋白通过磷酸化依赖性结合靶蛋白，调节广泛的生理过程，包括细胞增殖、细胞周期进展、凋亡程序抑制和神经元功能。为了产生研究工具来研究14-3-3在各种生物系统中的功能，并有可能开发14-3-3靶向治疗策略，我们开发了一种基于AlphaScreen技术的高通量分析，用于分离可以破坏14-3-3/客户蛋白相互作用的小分子。我们已经优化和小型化的分析，以适应384孔板格式的机器人操作。本提案的目标是在分子文库筛选中心网络中利用该试验分离可能干扰14-3-3与其客户蛋白相互作用的小分子化合物。