Induction of Differentiation into Vascular Endothelial Cells by FGF-2 on Cells Derived from Periodontal Ligament

FGF-2诱导牙周膜来源的细胞分化为血管内皮细胞

• 批准号: 24792336
• 负责人: SHIRAI Kaname
• 金额: $ 2.75万
• 依托单位: Health Sciences University of Hokkaido
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24792336/
• 关键词: 歯根膜; FGF-2; 血管内皮細胞

The purpose of the present study was to investigate whether the cells derived from periodontal ligament have the ability to differentiate a vascular endothelial cell by FGF-2. The TesPDL3 cells were expressed KDR/Flk-1 that VEGF-A receptors using anti KDR/Flk-1 antibodies. After the TesPDL3 cells were cultured with FGF-2, all RNA were collected and measured by RT-PCR. Expression of vWF and KDR/Flk-1 were increased both. The TesPDL3 cells were cultured with VEGF-A and showed significant cell growth compared with other cells. The conclusion that the TesPDL3 cells may have the potential to differentiate into endothelial cells by FGF-2, and undifferentiated cells derived periodontal ligament able to differentiate into vascular endothelial-cell-like cells.

本研究的目的是调查牙周膜来源的细胞是否具有通过FGF-2分化为血管内皮细胞的能力。用抗KDR/Flk-1抗体转染TesPDL 3细胞，表达VEGF-A受体KDR/Flk-1。在TesPDL 3细胞与FGF-2一起培养后，收集所有RNA并通过RT-PCR测量。vWF和KDR/Flk-1表达均增加。TesPDL 3细胞与VEGF-A一起培养，与其他细胞相比显示出显著的细胞生长。结论：TesPDL 3细胞可能具有通过FGF-2诱导分化为内皮细胞的潜能，牙周膜来源的未分化细胞能够分化为血管内皮细胞样细胞。

项目成果

Induction of Differentiation into Vascular Endothelial Cells by FGF-2 on Cells Derived from Periodontal Ligament

FGF-2诱导牙周膜来源的细胞分化为血管内皮细胞

• 发表时间: 2012
• 作者: K.Shirai;T.Hidaka;T.Ueyonahara;T.Kado;Y.Nakatsuka;Y.Furuichi
• 通讯作者: Y.Furuichi