FGF-2通过FGFR激活ERK信号通路诱导大体积脂肪组织再生的机制研究

传统的软组织重建手段以“移植”为核心，难以避免“拆东墙、补西墙”造成的供区损伤。本课题组从“再生”角度出发，发现脂肪不仅在生理状态下存在再生迭代，在脂肪移植、脂肪组织工程室等模型的诱导下，可以实现短时间内大体积（最高80ml）的再生。并在进一步的实验中，发现仅通过动态移植脂肪工程室中模型的渗出液，就可以诱发正常脂肪组织的大体积再生。借由另一临床中观察到的脂肪增生现象启发，查阅文献后展开预实验，我们发现FGF-2介导的ERK信号通路在此机制中起到重要作用。本研究拟围绕脂肪组织的再生诱导机制展开，采用细胞生物、蛋白生化、免疫组化/荧光等技术，重点研究FGF-2诱导脂肪组织短期内大体积再生的现象及其机制。为建立一种非手术（注射）诱导脂肪再生为核心的软组织重建技术奠定理论及实验基础。

Traditional breast reconstructive strategy focused on transplatation which inevitably results donor site malformation simultaneously. Hence we focus on the regeneration of adipose tissue. According to previous studies, adipose tissue renews itself physiologically about every 10 years and large amount of adipogenesis (up to 80ml) could be induced by tissue engineering chamber. What’s more, our experiment found that adipogenesis could be triggered even by dynamically transplantation of exudate in the tissue engineering chamber. Furthermore, clinical reports on adipose tissue hyperplasia after fat grafts inspired us, we reviewed the article and pilot experiments were done. We found that FGF-2/ERK pathway might play a crucial role in the regenerative process. Further studies will focus on the mechanism of FGF-2 induced adipogenesis. This mechanism could be the basement of a novel non-surgical soft tissue reconstructive strategy.