Estimation of Biocompatibility of Polymeric Materials without Living Cells

无活细胞的高分子材料的生物相容性评估

• 批准号: 24750216
• 负责人: GEMMEI Makoto
• 金额: $ 2.91万
• 依托单位: University of Toyama
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24750216/
• 关键词: 高分子機能材料; 生体適合性; 表面の水

Attenuated total reflectance (ATR) near infrared (NIR) and middle infrared (MIR) spectroscopes, which have the ability to observe the water-solid interface selectively, were constructed. Using ATR-NIR, a combination band (7000 cm-1) of OH symmetric and asymmetric stretching of water on hydrophilic (silanol) and hydrophobic (alkylated) solid surfaces was obtained. The ATR-NIR spectra indicated that the density of water on both the hydrophilic and hydrophobic surfaces increased in the range of 100 nm from the surface. Using ATR-MIR, amide I (1650 cm-1) and amide II (1550 cm-1) bands of the protein adsorbed on the surface were obtained. Structural change of the adsorbed protein on the hydrophobic surface was faster than that on the hydrophilic surface. A good correlation between the water structures and the structural changes of an adsorbed protein on the hydrophilic / hydrophobic surfaces was not observed.

构建了具有选择性观测水-固界面的衰减全反射(ATR)近红外(NIR)和中红外(MIR)光谱仪。利用ATR-NIR，得到了水在亲水性(硅醇)和疏水性(烷基化)固体表面上的OH对称和不对称伸展的结合带(7000 cm-1)。ATR-NIR光谱表明，在距表面100 nm的范围内，亲水和疏水表面上的水密度都增加了。利用ATR-MIR，得到了吸附在蛋白质表面的酰胺I(1650 cm-1)和酰胺II(1550 cm-1)谱带。吸附在疏水表面的蛋白质结构变化快于亲水表面。亲水/疏水表面上吸附的蛋白质的结构变化与水结构之间没有很好的相关性。

项目成果

Sum-frequency Generation Analyses on the Structure of Water at Amphoteric SAM-Liquid Water Interfaces.

两性 SAM-液态水界面处水结构的和频生成分析。

• 发表时间: 2014
• 期刊: Colloids Surfaces B: Biointerfaces
• 作者: Kouji Nomura;Tadashi Nakaji-Hirabayashi;Makoto Gemmei-Ide;Hiromi Kitano;Hidenori Noguchi;Kohei Uosaki
• 通讯作者: Kohei Uosaki

双性イオン型高分子中の再結晶化水

两性离子聚合物中的重结晶水

• 发表时间: 2012
• 作者: K. Shibata;S. Yamazoe and T. Wada;源明 誠，高谷 務之，堀岡 祐介，北野 博巳
• 通讯作者: 源明 誠，高谷 務之，堀岡 祐介，北野 博巳

Structure of water at zwitterionic copolymer film-liquid water interfaces as examined by the sum frequency generation method

• DOI: 10.1016/j.colsurfb.2013.08.051
• 发表时间: 2014-01-01
• 期刊: COLLOIDS AND SURFACES B-BIOINTERFACES
• 影响因子: 5.8
• 作者: Kondo, Takuya;Nomura, Kouji;Saruwatari, Yoshiyuki
• 通讯作者: Saruwatari, Yoshiyuki

材料表面の吸着タンパク質の構造と線維芽細胞の付着特性の相関

材料表面吸附蛋白结构与成纤维细胞粘附特性的相关性

• 发表时间: 2012
• 作者: S. Yamazoe;A. Kohori;H. Sakurai;T. Wada;源明 誠，上條 典昭，北野 博巳
• 通讯作者: 源明 誠，上條 典昭，北野 博巳

Sum frequency generation study on the structure of water in the vicinity of an amphoteric polymer brush.

• DOI: 10.1016/j.colsurfb.2011.11.012
• 发表时间: 2012-03
• 期刊: Colloids and surfaces. B, Biointerfaces
• 作者: T. Kondo;Makoto Gemmei-Ide;H. Kitano;K. Ohno;H. Noguchi;K. Uosaki