Analysis for the mechanisms of the centromeric cohesion by cohesin complex at meiosis I

我们发现，Moa 1是在减数分裂I期间建立姐妹动粒融合的不可或缺的因子，它可以与波罗样激酶（Plo 1）结合。通过多次分析，我发现Plo 1通过其激酶活性在姐妹动粒融合状态中起着至关重要的作用。此外，我发现SPO13基因，这已被证明是所需的芽殖酵母中的还原分离和波罗样激酶（CDC 5）的行为，可以抑制均等分离表型在moa1 δ细胞。这表明，尽管它们不共享序列同源性，SPO13是芽殖酵母中Moa1的功能同源物。

We found that Moa1, which is indispensable factor to establish fusion of sister kinetochores during meiosis I, can bind to Polo like kinase (Plo1). Through several analyses, I showed that Plo1 plays crucial role to make sister kinetochores fusion state via its kinase activity. In addition, I found that SPO13 gene, which has been shown to be required for reductional segregation in budding yeast and acts with Polo like kinase (CDC5), could suppress equational segregation phenotype in moa1 delta cells. This suggests that although they do not share the sequence homology, SPO13 is functional homolog of Moa1 in budding yeast.