Utilization strategy of trace element selenium in marine microalgae

海洋微藻微量元素硒的利用策略

• 批准号: 24770117
• 负责人: ARAIE Hiroya
• 金额: $ 1.83万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24770117/
• 关键词: セレン; トランスポーター; セレン含有化合物; Emiliania huxleyi; リポソーム; 微量元素; 輸送体; 円石藻; セレノシステイン

There is only trace amounts of essential element selenium (Se) in environment. Therefore, microalgae thought to possess specific Se transporter for efficient utilization of Se. I tried to identify this Se transporter. To date, there are no reports of transporters specific for selenite. This time, I aimed at building activity measurement system for the identification. Considering the fact that the nucleotide sequence of the algae is GC rich and the transporter is a membrane protein, I tried expression analysis by using a cell-free system. As a result, it was possible to synthesize a membrane protein by using liposomes as basis. In addition, I have found that there may be a similarity between Se compounds in various microalgae. Therefore, it is expected to identify Se transporter that uses algae that can be genetically engineered for knockdown assay.

人体必需元素硒（Se）在环境中仅存在微量。因此，认为微藻具有特异性硒转运蛋白，可有效利用硒。我试着识别这个硒转运体。到目前为止，还没有亚硒酸盐特异性转运蛋白的报道。这一次，我的目的是建立活动测量系统的识别。考虑到藻类的核苷酸序列富含GC并且转运蛋白是膜蛋白的事实，我尝试使用无细胞系统进行表达分析。结果，可以通过使用脂质体作为基础来合成膜蛋白。此外，我发现各种微藻中的Se化合物之间可能存在相似性。因此，预计将确定硒转运蛋白，使用藻类，可以基因工程敲低测定。