Multiscale motion analysis of membrane proteins at neuromuscular junction

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In this project, we engaged in developing the experimental technique to track internal motion and translational motion of membrane proteins at multi-time scale based on diffracted X-ray tracking method (DXT). In DXT, the internal motion of an individual single protein is monitored through the trajectory of the Laue spot from the nanocrystal labeled on the objective protein immobilized on the substrate surface. We improved a quality of the gold nanocrystal and optimized the experimental system to monitor ligand-induced motion of membrane protein, acetylcholine receptors, at several 10 micro seconds scale. Moreover, we examined a quantum dot as a DXT probe, that could be suited to track internal motion (DXT) and translational motion (fluorescence microscope) of membrane proteins.