small RNA mediated DNA methylation

反转录转座子约占基因组DNA的40%，众所周知，DNA甲基化介导的反转录转座子表达对哺乳动物至关重要。在雄性生殖细胞分化过程中，DNA甲基化被整体清除，然后以特定的方式获得DNA甲基化，包括反转录转座子。由于DNA甲基化导致基因抑制，因此DNA甲基化的位置特异性是至关重要的，然而，如何确定DNA甲基化的位置特异性仍然是一个难以捉摸的问题。通过遗传分析，Mili和Miwi2被认为是决定DNA甲基化位点特异性的重要基因，但其确切的分子机制仍不清楚。在这个项目中，我报告了MILI和MIWI2是如何影响反转录转座子DNA甲基化的位点特异性的

Retrotransposon occupies approximately 40% of genome DNA, and it is widely known that DNA methylation mediated retrotransposon repressions are critical for mammal. During male germ cell differentiation, DNA methylation is globally erased, followed by obtaining DNA methylation at site specific manner, including Retrotransposon. Since DNA methylation causes gene repression, site specificity of DNA methylation is critical, however, it remains elusive how site specificity of DNA methylation is determined. By genetic analysis, Mili and Miwi2 were identified as important genes for to determine site specificity for DNA methylation, however, the precise molecular mechanisms are largely unknown. In this project, I have reported how MILI and MIWI2 contribute to site specificity of DNA methylation for retrotransposon