small RNA mediated DNA methylation

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Retrotransposon occupies approximately 40% of genome DNA, and it is widely known that DNA methylation mediated retrotransposon repressions are critical for mammal. During male germ cell differentiation, DNA methylation is globally erased, followed by obtaining DNA methylation at site specific manner, including Retrotransposon. Since DNA methylation causes gene repression, site specificity of DNA methylation is critical, however, it remains elusive how site specificity of DNA methylation is determined. By genetic analysis, Mili and Miwi2 were identified as important genes for to determine site specificity for DNA methylation, however, the precise molecular mechanisms are largely unknown. In this project, I have reported how MILI and MIWI2 contribute to site specificity of DNA methylation for retrotransposon