PIWI-interacting RNA-guided somatic gene regulation in cell homeostasis and differentiation

PIWI相互作用RNA引导的体细胞基因调控细胞稳态和分化

• 批准号: RGPIN-2022-03780
• 负责人: Kakumani, PavanKumar
• 金额: $ 2.26万
• 依托单位: Memorial University of Newfoundland
• 依托单位国家: 加拿大
• 项目类别: Discovery Grants Program - Individual
• 财政年份: 2022
• 资助国家: 加拿大
• 起止时间: 2022-01-01 至 2023-12-31
• 项目状态: 已结题
• 来源: https://www.nserc-crsng.gc.ca/ase-oro/Details-Detailles_eng.asp?id=744083
• 关键词: PIWI; interacting; RNA; guided; somatic

In the late nineties, researchers uncovered a new cellular pathway in animals which is triggered by duplex RNA molecules and controls the flow of genetic information. This breakthrough discovery called RNA interference (RNAi) was acknowledged by the Nobel Committee in 2006 for its potential in the development of novel RNA-based technologies based on small non-coding RNAs (sncRNAs) that will be extremely useful for biological research and industrial application. My research group will investigate the role of sncRNA named PIWI-interacting RNA (piRNA) in gene regulation using human and mouse cell lines, including those with regenerative capacity to understand their complex mechanisms defining cellular characteristics and function. I believe that this innovative research program will contribute to unveil the importance of piRNAs in determining cellular identity and support the use of piRNAs as molecular tools in research laboratories and industries focused on life science and biomedicine.

上世纪90年代末，研究人员在动物体内发现了一种由双链RNA分子触发并控制遗传信息流动的新细胞途径。这项突破性的发现被称为RNA干扰（RNAi），并于2006年获得诺贝尔委员会的认可，因为它在开发基于小非编码RNA （sncRNAs）的新型RNA技术方面具有潜力，这将对生物学研究和工业应用非常有用。我的研究小组将研究名为piwi相互作用RNA （piRNA）的sncRNA在人类和小鼠细胞系（包括具有再生能力的细胞系）基因调控中的作用，以了解其定义细胞特征和功能的复杂机制。我相信这个创新的研究项目将有助于揭示pirna在决定细胞身份方面的重要性，并支持pirna作为分子工具在研究实验室和专注于生命科学和生物医学的行业中的使用。