Accumulation of reactive oxygen species in soybean under waterlogging

点击翻译按钮获取中文摘要

We investigated the accumulation of superoxide (O2-) and hydrogen peroxide (H2O2) of soybean roots. Soybeans grown for 1 week were affected by waterlogging for 1 week. One day after removing the stress, tap roots were used for the staining of O2- and H2O2. O2- staining was observed in the middle region of a tap root. The accumulation of H2O2 was detected on plasma membrane in a cortical cell of a tap root. Thus, waterlogging induces the excess accumulation of O2- and O2- derived H2O2 in a cortical cell of a tap root.We also investigated the growth and yields 98 days after sowing of soybean, which was subjected by waterlogging in an early growth stage. The shoot and root growth, grain and nodule weight, chlorophyll contents and photosynthesis rate of fully uppermost leaves decreased compared with those of the control. These results suggest that waterlogging in the early growth stage induces the decrease in growth and the earlier etiolation of leaves in the late growth stage.