Relationship between chlorotic symptom expression and regulation of host-gene expression by Cucumber mosaic virus infection

我们比较了三种不同程度的黄瓜花叶病毒（CMV）侵染的组织的转录组。CMV接种似乎对转录表达谱有相似的影响，只是不同CMV之间的表达量不同。基因本体论分析显示，叶绿体和光合作用相关基因（CPRGs）在褪绿组织中下调。CPRG下调水平与黄化程度相关。为了阐明CPRGs下调导致黄化的说法，通过引入拟南芥GLK转录因子，产生了CPRGs表达上调的转基因烟草。接种cmv后，glk -烟草出现褪绿症状。感染glk -烟草的CPRGs mRNA表达量低于健康烟草，表明过表达atGLK几乎不影响黄化表达。

We compared the transcriptomes of chlorotic tissues infected with three Cucumber mosaic virus (CMV) strains with diverse chlorosis severity. CMV inoculation appeared to have similar effects on the transcriptional expression profiles, and only the magnitude of expression differed among the different CMVs. Gene ontology analysis revealed chloroplast- and photosynthesis-related genes (CPRGs) were down-regulated in the chlorotic tissues. The level of CPRG down-regulation was correlated with the severity of chlorosis.To elucidate the suggestion that the down-regulation of CPRGs caused chlorosis, transgenic tobacco that CPRGs expression was up-regulated by introduction of the Arabidopsis GLK transcriptional factor was generated. When GLK-tobacco were inoculated with CMVs, chlorotic symptoms appeared. Amounts of CPRGs mRNA in infected GLK-tobaccos were fewer than those of healthy tobacco, indicating that over-expression of atGLK hardly affected chlorosis expression.