Relationship between chlorotic symptom expression and regulation of host-gene expression by Cucumber mosaic virus infection

黄瓜花叶病毒感染褪绿症状表达与宿主基因表达调控的关系

• 批准号: 24780041
• 负责人: MOCHIZUKI Tomofumi
• 金额: $ 2.91万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780041/
• 关键词: キュウリモザイクウイルス; 退緑症状; 光合成関連遺伝子; トランスクリプトーム; 葉緑体; 外被タンパク質; タバコ; クロロシス

We compared the transcriptomes of chlorotic tissues infected with three Cucumber mosaic virus (CMV) strains with diverse chlorosis severity. CMV inoculation appeared to have similar effects on the transcriptional expression profiles, and only the magnitude of expression differed among the different CMVs. Gene ontology analysis revealed chloroplast- and photosynthesis-related genes (CPRGs) were down-regulated in the chlorotic tissues. The level of CPRG down-regulation was correlated with the severity of chlorosis.To elucidate the suggestion that the down-regulation of CPRGs caused chlorosis, transgenic tobacco that CPRGs expression was up-regulated by introduction of the Arabidopsis GLK transcriptional factor was generated. When GLK-tobacco were inoculated with CMVs, chlorotic symptoms appeared. Amounts of CPRGs mRNA in infected GLK-tobaccos were fewer than those of healthy tobacco, indicating that over-expression of atGLK hardly affected chlorosis expression.

我们比较了不同黄化程度的黄瓜花叶病毒（CMV）株系感染黄化组织的转录组。CMV接种似乎对转录表达谱有类似的影响，不同CMV之间只有表达的幅度不同。基因本体分析表明，叶绿体和光合作用相关基因（CPRGs）在失绿组织中表达下调。为了阐明CPRGs表达下调导致叶片失绿的可能性，本研究通过导入拟南芥GLK转录因子，构建了CPRGs表达上调的转基因烟草。GLK烟草接种CMVs后，出现褪绿症状。感染GLK的烟草中CPRGs mRNA的量少于健康烟草，表明atGLK的过度表达几乎不影响失绿表达。

项目成果

CMVによるモザイク病徴の発現メカニズム

CMV引起花叶症状的机制

• 发表时间: 2013
• 作者: MOCHIZUKI Tomofumi;OGATA Yoshiyuki;HIRATA Yuki;OHKI T. Satoshi.;山崎稜太，望月知史，大木 理;関根健太郎;望月知史
• 通讯作者: 望月知史

Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with the Cucumber mosaic virus

感染黄瓜花叶病毒的烟草植物花叶叶中与失绿严重程度相关的定量转录变化

• 发表时间: 2014
• 期刊: Molecular Plant Pathology
• 影响因子: 4.9
• 作者: MOCHIZUKI Tomofumi;OGATA Yoshiyuki;HIRATA Yuki;OHKI T. Satoshi
• 通讯作者: OHKI T. Satoshi

Quantitative transcriptional changes associated with chlorosis severity in mosaic leaves of tobacco plants infected with Cucumber mosaic virus

• DOI: 10.1111/mpp.12081
• 发表时间: 2014-04-01
• 期刊: MOLECULAR PLANT PATHOLOGY
• 影响因子: 4.9
• 作者: Mochizuki, Tomofumi;Ogata, Yoshiyuki;Ohki, Satoshi T.
• 通讯作者: Ohki, Satoshi T.

キュウリモザイクウイルスに感染したタバコにおけるtranscriptome・small RNAome解析

黄瓜花叶病毒感染烟草的转录组/小 RNA 组分析

• 发表时间: 2014
• 作者: MOCHIZUKI Tomofumi;OGATA Yoshiyuki;HIRATA Yuki;OHKI T. Satoshi.;山崎稜太，望月知史，大木 理
• 通讯作者: 山崎稜太，望月知史，大木 理

毒性が異なるキュウリモザイクウイルス変異株が感染したタバコモザイク葉退緑組織におけるトランスクリプトームの比較解析

不同毒力黄瓜花叶病毒突变体感染烟草花叶失绿组织转录组比较分析

• 发表时间: 2013
• 作者: Mizumoto;H.;Nakamura;I.;Shimomoto;Y.;Sawada;H.;Tomita;R.;Sekine;K.-T.;Kiba;A.;Nishiguchi;M.;Kobayashi;K.;and Hikichi;Y.;望月知史・尾形善之・平田裕樹・大木理
• 通讯作者: 望月知史・尾形善之・平田裕樹・大木理