Functional analysis of alpha-1,3-glucanase: the enzyme participates in fungal cell-wall hydrolysis

α-1,3-葡聚糖酶的功能分析：该酶参与真菌细胞壁水解

• 批准号: 24780085
• 负责人: YANO Shigekazu
• 金额: $ 2万
• 依托单位: Yamagata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780085/
• 关键词: α-1,3-グルカナーゼ; 細胞壁; α-1,3-グルカン; 真菌細胞壁; 糸状菌細胞壁

Bacillus circulans KA-304 alpha-1,3-glucanase (Agl-KA) is a multi-domain enzyme, and it includes N-terminal DS1, CB6, DS2, UCD, and C-terminal catalytic domain. To clarify the role of these domains, we constructed several domain deletion enzymes and GFP-fusion proteins. As the results, DS1, CB6, and DS2 domain bound to alpha-1,3-glucan and fungal cell-wall, and that binding efficiency was increased by combined action. The findings indicate that DS1, CB6, and DS2 are novel alpha-1,3-glucan binding domain.In order to obtain the further information on structure and function of alpha-1,3-glucanases, we purified and characterized a novel alpha-1,3-glucanase (Agl-FH1) of Paenibacillus glycanilyticus FH11. Although the properties of Agl-FH1 were almost the same as those of Agl-KA, the amino acid sequence of catalytic domain of Agl-FH1 showed approximately 20% identity to that of Agl-KA. The results may be beneficial to clarify the catalytic mechanism of the enzyme in the future.

环状芽孢杆菌KA-304α-1，3-葡聚糖酶(AGL-KA)是一个多结构域的酶，它包括N-端的DS1、CB6、DS2、UCD和C-端的催化区。为了阐明这些结构域的作用，我们构建了几个结构域缺失酶和GFP融合蛋白。结果表明，DS1、CB6和DS2结构域与α-1，3-葡聚糖和真菌细胞壁结合，并且结合效率在联合作用下提高。为了进一步了解α-1，3-葡聚糖酶的结构和功能，我们纯化并鉴定了一种新的α-1，3-葡聚糖酶(AGL-FH1)。尽管AGL-FH1的性质与AGL-KA基本相同，但AGL-FH1的催化区氨基酸序列与AGL-KA的同源性约为20%。这一结果可能有助于进一步阐明该酶的催化机理。

项目成果

糸状菌細胞壁溶解に寄与するPaenibacilllus sp. No.11株由来α-1,3-グルカナーゼの解析

类芽孢杆菌 No.11 菌株中有助于丝状真菌细胞壁裂解的 α-1,3-葡聚糖酶分析

• 发表时间: 2013
• 作者: 矢野成和;ワサナスヨータ;藤本寛子;立木隆;若山守
• 通讯作者: 若山守

Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus.

• DOI: 10.1371/journal.pone.0054893
• 发表时间: 2013
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yoshimi A;Sano M;Inaba A;Kokubun Y;Fujioka T;Mizutani O;Hagiwara D;Fujikawa T;Nishimura M;Yano S;Kasahara S;Shimizu K;Yamaguchi M;Kawakami K;Abe K
• 通讯作者: Abe K

Characterization of α-1,3-glucanase isozyme from Paenibacillus glycanilyticus FH11 in a new subgroup of family 87 α-1,3-glucanase.

87 α-1,3-葡聚糖酶家族新亚组中来自溶糖类芽孢杆菌 FH11 的 α-1,3-葡聚糖酶同工酶的表征。

• DOI: 10.1016/j.jbiosc.2014.03.008
• 发表时间: 2014
• 期刊: Journal of bioscience and bioengineering
• 影响因子: 2.8
• 作者: Wasana Suyotha;Shigekazu Yano;T. Itoh;H. Fujimoto;T. Hibi;T. Tachiki;M. Wakayama
• 通讯作者: M. Wakayama

細菌型α-1,3-グルカナーゼのドメイン構造

细菌 α-1,3-葡聚糖酶的结构域结构

• 发表时间: 2013
• 作者: 矢野成和;ワサナスヨータ;立木隆;若山守
• 通讯作者: 若山守

Domain Structure and Function of α-1,3-Glucanase from Bacillus circulans KA-304, an Enzyme Essential for Degrading Basidiomycete Cell Walls

• DOI: 10.1271/bbb.120900
• 发表时间: 2013-03
• 期刊: Bioscience, Biotechnology, and Biochemistry
• 作者: Wasana Suyotha;Shigekazu Yano;K. Takagi;Nopakarn Rattanakit-Chandet;T. Tachiki;M. Wakayama