Novel influenza A virus proteins translated from PA mRNA

PA mRNA 翻译的新型甲型流感病毒蛋白

• 批准号: 24780296
• 负责人: MURAMOTO Yukiko
• 金额: $ 3万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780296/
• 关键词: インフルエンザウイルス

I identified novel PA-related proteins in influenza A virus-infected cells. These proteins are translated from the eleventh and thirteenth in-frame AUG codons in the PA mRNA and are, therefore, N-terminally truncated forms of PA, which we named PA-N155 and PA-N182, respectively. The eleventh and thirteenth AUG codons are highly conserved among influenza A viruses, and the PA-N155 and PA-N182 proteins were detected in cells infected with various influenza A viruses isolated from different host species, suggesting the expression of these N-truncated PAs is universal in nature among influenza A viruses. These N-truncated PAs did not show polymerase activity when expressed together with PB1 and PB2; however, mutant viruses lacking the N-truncated PAs replicated more slowly in cell culture and had lower pathogenicity in mice than did wild-type virus. These results suggest that these novel PA-related proteins likely possess important functions in the replication cycle of influenza A virus.

我在A型流感病毒感染的细胞中鉴定了新的PA相关蛋白。这些蛋白质从PA mRNA中的第十一和第十三框内AUG密码子翻译，因此是PA的N-末端截短形式，我们分别将其命名为PA-N155和PA-N182。第十一和第十三AUG密码子在甲型流感病毒中高度保守，并且在用从不同宿主物种分离的各种甲型流感病毒感染的细胞中检测到PA-N155和PA-N182蛋白，表明这些N-截短的PA的表达在甲型流感病毒中是普遍的。这些N-截短的PA与PB 1和PB 2一起表达时没有显示聚合酶活性;然而，缺乏N-截短的PA的突变病毒在细胞培养中复制更慢，并且在小鼠中的致病性比野生型病毒低。这些结果表明，这些新的PA相关蛋白可能在甲型流感病毒的复制周期中具有重要的功能。

项目成果

Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities

感染不同H5N1高致病性禽流感病毒的非人灵长类动物不同疾病严重程度的基因表达动态比较

• 发表时间: 2014
• 作者: 土赤忍;大松勉;平田哲兵;城間康;片山幸枝;大場真巳;佐々悠木子;長井誠;古谷哲也;玉城史朗;水谷哲也;Yukiko Muramoto
• 通讯作者: Yukiko Muramoto

Integrated Clinical, Pathologic, Virologic, and Transcriptomic Analysis of H5N1 Influenza Virus-Induced Viral Pneumonia in the Rhesus Macaque

• DOI: 10.1128/jvi.00365-12
• 发表时间: 2012-06-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Shinya, Kyoko;Gao, Yuwei;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Identification of Novel Influenza A Virus Proteins Translated from PA mRNA

• DOI: 10.1128/jvi.02656-12
• 发表时间: 2013-03-01
• 期刊: JOURNAL OF VIROLOGY
• 影响因子: 5.4
• 作者: Muramoto, Yukiko;Noda, Takeshi;Kawaoka, Yoshihiro
• 通讯作者: Kawaoka, Yoshihiro

Comparison of gene expression dynamics in non-human primates infected with different H5N1 highly pathogenic avian influenza viruses between different disease severities.

感染不同H5N1高致病性禽流感病毒的非人灵长类动物不同疾病严重程度之间的基因表达动态比较。

• 发表时间: 2014
• 作者: Yukiko Muramoto;Jason E. Shoemaker;Le Thi Quynh Mai;Yasushi Itoh;Daisuke Tamura;Ryuta Uraki;Yuko Sakai-Tagawa;Hirotaka Imai;Ryo Takano;Eiryo Kawakami;Kiyoko Okamoto;Hirohito Ishigaki;Takeshi Noda;Satoshi Fukuyama;Kazumasa Ogasawara;Hiroaki
• 通讯作者: Hiroaki