Analysis of CDV quasispecies and establishement of the reverse genetics system

CDV准种分析及反向遗传学体系的建立

• 批准号: 24780303
• 负责人: YOSHIKAWA Tomoki
• 金额: $ 3万
• 依托单位: National Institute of Infectious Diseases
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780303/
• 关键词: イヌジステンパーウイルス; ウイルス; 犬ジステンパー; リバースジェネティクス

There was a canine distemper outbreak, which is generally known to be occurred in carnivore happened in the monkey colony in an animal quarantine. Hence to understand the pathogenesis, the sequences from the in vitro isolates and from these of clinical specimens were determined and analyzed by utilizing next-generation sequencing technique. In addition, establishment of the reverse genetics system based on the S-CDV sequence, which enable to produce the infectious particles from the plasmids were tried.

在动物检疫的猴群中发生了犬瘟热疫情，这通常发生在食肉动物中。因此，为了了解其发病机制，我们利用新一代测序技术对体外分离株和临床标本的序列进行了测定和分析。此外，还尝试建立基于S-CDV序列的反向遗传系统，使质粒能够产生感染性颗粒。

项目成果

Imported case of acute respiratory tract infection associated with a member of species nelson bay orthoreovirus.

• DOI: 10.1371/journal.pone.0092777
• 发表时间: 2014
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Yamanaka A;Iwakiri A;Yoshikawa T;Sakai K;Singh H;Himeji D;Kikuchi I;Ueda A;Yamamoto S;Miura M;Shioyama Y;Kawano K;Nagaishi T;Saito M;Minomo M;Iwamoto N;Hidaka Y;Sohma H;Kobayashi T;Kanai Y;Kawagishi T;Nagata N;Fukushi S;Mizutani T;Tani H;Taniguchi S;Fukuma A;Shimojima M;Kurane I;Kageyama T;Odagiri T;Saijo M;Morikawa S
• 通讯作者: Morikawa S

A seroepidemiologic study of Reston ebolavirus in swine in the Philippines.

• DOI: 10.1186/1746-6148-8-82
• 发表时间: 2012-06-18
• 期刊: BMC veterinary research
• 影响因子: 2.6
• 作者: Sayama Y;Demetria C;Saito M;Azul RR;Taniguchi S;Fukushi S;Yoshikawa T;Iizuka I;Mizutani T;Kurane I;Malbas FF Jr;Lupisan S;Catbagan DP;Animas SB;Morales RG;Lopez EL;Dazo KR;Cruz MS;Olveda R;Saijo M;Oshitani H;Morikawa S
• 通讯作者: Morikawa S

The First Identification and Retrospective Study of Severe Fever With Thrombocytopenia Syndrome in Japan

• DOI: 10.1093/infdis/jit603
• 发表时间: 2014-03-15
• 期刊: JOURNAL OF INFECTIOUS DISEASES
• 影响因子: 6.4
• 作者: Takahashi, Toru;Maeda, Ken;Saijo, Masayuki
• 通讯作者: Saijo, Masayuki

Canine Distemper Virus Associated with a Lethal Outbreak in Monkeys Readily Adapted to Use Human Receptors

犬瘟热病毒与易于使用人类受体的猴子的致命爆发有关

• 发表时间: 2013
• 期刊: J Virol
• 影响因子: 5.4
• 作者: Sakai;K. Yoshikawa;T. Seki;F. Fukushi;S. Tahara;M. Nagata;N. Ami;Y. Mizutani;T. Kurane;I. Yamaguchi;R. Hasegawa;H. Saijo;M. Komase;K. Morikawa;S. Takeda;M.
• 通讯作者: M.

IPS-1-dependent innate immune response is indispensable for limiting the SARS-CoV propagation in airway epithelial cell

IPS-1依赖性先天免疫反应对于限制SARS-CoV在气道上皮细胞中的传播是必不可少的

• 发表时间: 2012
• 作者: Tomoki Yoshikawa;Shuetsu Fukushi;Chien-T K Tseng;Clarence J Peters
• 通讯作者: Clarence J Peters