Elucidation of the molecular network controlling sperm acrosome formation in mammals

阐明控制哺乳动物精子顶体形成的分子网络

• 批准号: 24780327
• 负责人: KANEMORI Yoshinori
• 金额: $ 2.91万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24780327/
• 关键词: マウス; 精子形成; 受精; 先体反応; 精子形成過程

Mammalian sperm possess a cap-shaped, exocytotic vesicle, acrosome that is located at the anterior part of the nucleus. ACRBP/sp32, is an acrosomal protein, initially identified as a binding protein for the precursor (proACR) of sperm serine protease ACR. Unlike other mammalian ACRBPs, two forms of Acrbp mRNA, wild-type ACRBP-W and variant Acrbp-V5 mRNAs, were generated by alternative splicing of Acrbp in the mouse. In this study, we produced knockout mice lacking ACRBP to uncover the role of ACRBP in spermatogenesis and fertilization. These results demonstrate that ACRBP-V5 palys key role in condensation and subsequent spread of acrosomal proteins in the acrosome during spermiogenesis. It is also suggested that ACRBP-W may function in the retention of proACR in the acrosome until after sperm undergo acrosomal exocytosis.

哺乳动物精子具有帽状的胞吐囊，顶体位于核的前部。ACRBP/sp32是一种顶体蛋白，最初被鉴定为精子丝氨酸蛋白酶ACR的前体（proACR）的结合蛋白。与其他哺乳动物ACRBP不同，Acrbp mRNA的两种形式，野生型ACRBP-W和变体Acrbp-V5 mRNA，是通过小鼠中Acrbp的选择性剪接产生的。在这项研究中，我们产生了敲除小鼠缺乏ACRBP揭示ACRBP在精子发生和受精的作用。这些结果表明，ACRBP-V5在精子发生过程中的顶体蛋白的凝聚和随后的传播中发挥关键作用。这也表明，ACRBP-W可能在顶体中保留前ACR的功能，直到精子经历顶体胞吐。

项目成果

Functional role of mouse ACRBP/sp32 in spermatogenesis and fertilization

小鼠 ACRBP/sp32 在精子发生和受精中的功能作用

• 发表时间: 2012
• 作者: Ryu;J. -H.;Sudo;M.;Kanemori;Y.;Kashiwabara;S.;Baba;T.
• 通讯作者: T.

精子アクロソームタンパク質ACRBPとACRのダブル欠損マウスの解析

双缺陷小鼠精子顶体蛋白 ACRBP 和 ACR 分析

• 发表时间: 2013
• 作者: 高嶋明日登;兼森芳紀;首藤舞;康宇鎮;馬場忠
• 通讯作者: 馬場忠

Proacrosin-binding protein ACRBP/sp32 regulates acrosome formation during mouse spermiogenesis.

顶体蛋白原结合蛋白 ACRBP/sp32 在小鼠精子发生过程中调节顶体形成。

• 发表时间: 2013
• 作者: Kanemori;Y.;Sudo;M.;Koga;Y.;Okabe;M.;and Baba;T.
• 通讯作者: T.

Two functional forms of ACRBP/sp32 are produced by pre-mRNA alternative splicing in mouse spermatogenesis

ACRBP/sp32 的两种功能形式是由小鼠精子发生过程中前体 mRNA 选择性剪接产生的

• 发表时间: 2013
• 作者: Sudo;M.;Kanemori;Y.;Ryu;JH.;Niida-Araida;Y.;Kodaira;K.;Takenaka;M.;Kohno;N.;Sugiura;S.;Kashiwabara;SI.;and Baba;T.
• 通讯作者: T.

Two Functional Forms of ACRBP/sp32 Are Produced by Pre-mRNA Alternative Splicing in the Mouse

• DOI: 10.1095/biolreprod.112.107425
• 发表时间: 2013-04-01
• 期刊: BIOLOGY OF REPRODUCTION
• 影响因子: 3.6
• 作者: Kanemori, Yoshinori;Ryu, Jin-Hyeob;Baba, Tadashi
• 通讯作者: Baba, Tadashi