Molecular mechanism of meiotic arrest through Mos-MAPK pathway in mouse oocytes

Mos-MAPK通路抑制小鼠卵母细胞减数分裂的分子机制

• 批准号: 22780299
• 负责人: KANEMORI Yoshinori
• 金额: $ 2.58万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22780299/
• 关键词: 発生; 分化制御; マウス; 減数分裂; シグナル伝達; MAPキナーゼ; 卵子; MAPK

Ovulated oocytes are arrested at the metaphase of second meiotic division (metaphase-II). In this study, we showed possible role of mitogen-and stress-activated kinase 1, MSK1 known as a downstream kinase of the Mos-MAPK pathway, in the mouse oocytes. MSK1 exhibited the ability to phosphorylate four Ser/Thr residues of meiotic cell-cycle regulator EMI2. The phosphorylation was required for up-regulation of the EMI2 activity in the oocytes. These results suggested that mouse MSK1 may play a key role in the metaphase-II arrest through phosphorylation of EMI2.

排卵的卵母细胞在第二次减数分裂中期（中期- ii）被阻滞。在这项研究中，我们展示了丝裂原和应激激活激酶1 （MSK1）在小鼠卵母细胞中的可能作用，MSK1被称为Mos-MAPK途径的下游激酶。MSK1能够磷酸化减数分裂细胞周期调节因子EMI2的4个丝氨酸/苏氨酸残基。磷酸化是上调卵母细胞中EMI2活性所必需的。这些结果表明，小鼠MSK1可能通过磷酸化EMI2在中期ii阻滞中发挥关键作用。

项目成果

(These authors contributed equally) Possible involvement of mitogen-and stress-activated protein kinase 1, MSK1

（这些作者的贡献相同）有丝分裂原和应激激活蛋白激酶 1、MSK1 可能参与其中

• 发表时间: 2011
• 期刊: in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes. Developmental Biology
• 作者: Miyagaki;Y.;Kanemori;Y. and Baba;T
• 通讯作者: T

Possible involvement of mitogen- and stress-activated protein kinase 1, MSK1, in metaphase-II arrest through phosphorylation of EMI2 in mouse oocytes.

• DOI: 10.1016/j.ydbio.2011.08.021
• 发表时间: 2011-11
• 期刊: Developmental biology
• 影响因子: 2.7
• 作者: Yu Miyagaki;Yoshinori Kanemori;T. Baba

Dynamic Regulation of Emi2 by Emi2-Bound Cdk1/Plk1/CK1 and PP2A-B56 in Meiotic Arrest of Xenopus Eggs

• DOI: 10.1016/j.devcel.2011.06.029
• 发表时间: 2011-09-13
• 期刊: DEVELOPMENTAL CELL
• 影响因子: 11.8
• 作者: Isoda, Michitaka;Sako, Kosuke;Sagata, Noriyuki
• 通讯作者: Sagata, Noriyuki

Direct phosphorylation of EMI2 by MSK1 in the Mos-MAPK pathway during oocyte maturation

卵母细胞成熟过程中 Mos-MAPK 通路中 MSK1 直接磷酸化 EMI2

• 发表时间: 2011
• 作者: Miyagaki;Y.;Kanemori;Y. and Baba;T
• 通讯作者: T

Possible involvement of mitogen-and stress-activated protein kinase 1, MSK1

丝裂原和应激激活蛋白激酶 1 (MSK1) 可能参与其中

• 发表时间: 2011
• 作者: Miyagaki;Y.;Kanemori;Y. and Baba;T
• 通讯作者: T