The study of the role of ezrin in a model mouse of Parkinson's disease

Ezrin、Radioxin和Moesin统称为ERM蛋白。我们发现只有微量注射6-羟基多巴胺(6-OHDA)的帕金森病(PD)模型小鼠的海马区Ezrin才增加。这些结果表明，过量的Ezrin可能与视觉空间记忆损害有关。我们还研究了Ezrin在Ezrin基因敲除(EKD)小鼠胚胎培养的皮质神经元中的作用。我们证明，从EKD小鼠胚胎中制备的培养的皮质神经元表现出神经再生的障碍，而不是轴突和轴突的生长。此外，我们观察到RhoA活性和肌球蛋白轻链2(MLC2)的磷酸化增加。此外，Y-27632的治疗挽救了EKD神经元神经发生的异常。这些数据表明，Ezrin通过下调RhoA/Rho激酶/MLC2通路在培养的皮质神经元的神经发生中发挥了新的作用。

Ezrin, radixin and moesin are collectively known as ERM proteins. We found that ezrin was only increased in the hippocampus of the microinjected mice with 6-hydroxydopamine (6-OHDA), a model mouse of Parkinson's disease (PD). These results suggest that excessive ezrin may be related to visuo-spatial memory impairments. We also performed studies on the roles of ezrin in the cultured cortical neurons prepared from the ezrin knockdown (EKD) mice embryo. We demonstrated that the cultured cortical neurons prepared from the EKD mice embryo exhibited impairment of neuritogenesis, not neurite and axon outgrowth. Moreover, we observed increased RhoA activity and phosphorylation of myosin light chain 2 (MLC2). In addition, treatment of Y-27632 rescued the abnormalities in neuritogenesis in the EKD neurons. These data suggest a novel role of ezrin in the neuritogenesis of the cultured cortical neurons via down-regulation of RhoA/Rho kinase/MLC2 pathway.