development of the universal inducing agent for induction of antivirus cytotoxic T lymphocytes

细胞毒性T淋巴细胞（CTL）具有清除体内病毒感染细胞或癌细胞的作用。利用猿猴病毒40 （SV40）的VP1衣壳蛋白，研制了一种新的抗病毒CTL诱导剂。将HLA-A*02:01抗甲型流感病毒基质蛋白1 (M1) CTL肽GILGFVFTL （FMP:58 ~ 66）插入暴露在SV40 VP1衣壳表面的VP1 DE或HI环区。纯化的FMP:58-66插入重组VP1免疫HLA-A2转基因小鼠。正如预期的那样，免疫小鼠淋巴细胞中含有特异性抵抗FMP:58-66的CTL。结果表明，基于vp1的通用诱导剂可以在不需要佐剂的情况下诱导靶向CTL。通过在实验水平上进一步验证基于vp1的药物如何有效诱导靶向CTL的分子机制和对体内癌症的疗效，将开发出基于vp1的药物，用于从人体中清除病毒感染的细胞或癌细胞。

Cytotoxic T lymphocyte (CTL) has a role to eliminate virus-infected cells or cancer cells from the body. We developed novel universal inducing agent for antiviral CTL production using VP1 capsid protein of simian virus 40 (SV40). CTL peptide of HLA-A*02:01 against influenza A virus matrix protein 1 (M1), GILGFVFTL (FMP:58-66), was inserted into DE or HI loop region of VP1 which is exposed on the surface of the SV40 VP1 capsid. Purified FMP:58-66 inserted recombinant VP1 was immunized to HLA-A2 transgenic mice. As expected, lymphocyte of immunized mice contained CTL that responded against FMP:58-66 specifically. It indicated that VP1-based universal inducing agent could induce aimed CTL without need of adjuvant. By validating the molecular mechanism how VP1-based agent efficiently induce aimed CTL and the efficacy for cancer in vivo in experimental level more, VP1-based agent will be developed to eliminate virus infected cells or cancer cells from the human body for practical use.