Mechanism of regulated exocytosis of neuropeptides by Rab3/27

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Rab-family G proteins control membrane traffic which is essential for cell survival and function. In particular, Rab3 and Rab27 are critical factors for regulated-exocytosis which is used for secretion. However, the mechanism which regulates Rab3/Rab27 activities is largely unknown, because we have very little information about when and where the activity of Rab3 or Rab27 changes during the process of membrane fusion. In this study, we newly developed FRET sensors for Rab3 and Rab27. The dynamic range of Rab3 sensor is 73.0%. The dynamic range of Rab27 sensor is 106,3%. Both dynamic ranges are larger than the minimum criteria for cellular FRET imaging. We obtained some novel findings using these FRET sensors.