Analysis of the functional roles of Periplakin in homeostasis

我们寻找周边蛋白在体内平衡中的功能作用。在正常和胆汁淤积小鼠肝脏中筛选periplakin相互作用蛋白。在胆汁淤积性肝脏中发现了几种候选者。基因和蛋白表达谱也被检测。围血小板功能丧失对胆汁淤积过程中几种肝脏分子表达的影响；然而，这种差异与遗传背景和育种条件高度相关。这些结果表明，periplakin在胆汁淤积性肝脏中的作用可能是有限的；否则，小鼠肝脏可能存在外源蛋白的功能冗余。有趣的是，与性别匹配的野生型幼崽相比，无periplakin小鼠的空腹血糖水平可重复降低。因此，提示periplakin在空腹血糖代谢中的功能作用。

We sought the functional roles of periplakin in homeostasis. Periplakin-interacting proteins were screened in normal- and cholestatic mouse liver. Several candidates were identified in cholestatic liver. The gene- and protein expression profiles were also examined. The loss of periplakin function influenced on the expressions of several hepatic molecules during cholestasais; however, the difference were highly susceptible to the genetic background and breeding conditions. These results indicate that the roles of periplakin in cholestatic liver might be limited; otherwise, the functional redundancy for periplakin may exist in mouse liver. Interestingly, the fasting blood glucose levels were reproducibly lowered in periplakin-null mice compared to the gender-matched wild-type littermates. Thus, a functional role of periplakin in fasting blood glucose metabolism was suggested.