Analysis of the functional roles of Periplakin in homeostasis

Periplakin 在体内平衡中的功能作用分析

• 批准号: 24790314
• 负责人: ITO Shinji
• 金额: $ 2.83万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24790314/
• 关键词: Ｐｅｒｉｐｌａｋｉｎ; 肝臓; 胆汁鬱滞; 糖代謝; Periplakin

We sought the functional roles of periplakin in homeostasis. Periplakin-interacting proteins were screened in normal- and cholestatic mouse liver. Several candidates were identified in cholestatic liver. The gene- and protein expression profiles were also examined. The loss of periplakin function influenced on the expressions of several hepatic molecules during cholestasais; however, the difference were highly susceptible to the genetic background and breeding conditions. These results indicate that the roles of periplakin in cholestatic liver might be limited; otherwise, the functional redundancy for periplakin may exist in mouse liver. Interestingly, the fasting blood glucose levels were reproducibly lowered in periplakin-null mice compared to the gender-matched wild-type littermates. Thus, a functional role of periplakin in fasting blood glucose metabolism was suggested.

我们寻求的功能作用的periplakin的稳态。在正常和胆汁淤积的小鼠肝脏中筛选Periplakin相互作用蛋白。在胆汁淤积性肝脏中确定了几种候选物。基因和蛋白质表达谱也进行了检查。periplakin功能的丧失影响了胆汁淤积时几种肝脏分子的表达，但这种差异受遗传背景和饲养条件的影响。这些结果表明，periplakin在胆汁淤积性肝脏中的作用可能是有限的，否则，periplakin在小鼠肝脏中可能存在功能冗余。有趣的是，与性别匹配的野生型同窝出生小鼠相比，periplakin-null小鼠的空腹血糖水平可再现地降低。因此，提出了periplakin在空腹血糖代谢中的功能作用。

项目成果

Cholestasis induces reversible accumulation of periplakin in mouse liver.

• DOI: 10.1186/1471-230x-13-116
• 发表时间: 2013-07-13
• 期刊: BMC gastroenterology
• 影响因子: 2.4
• 作者: Ito S;Satoh J;Matsubara T;Shah YM;Ahn SH;Anderson CR;Shan W;Peters JM;Gonzalez FJ
• 通讯作者: Gonzalez FJ