Synthesis of [18F]labelled proteins using cell-free system

首先，我们使用无细胞蛋白质合成系统合成了[19 F]-白细胞介素-8（IL-8），其含有非标记的氟脯氨酸而不是脯氨酸。我们证实可以获得纯度和丰度高的[19 F]-IL-8。保留了[19 F] IL-8对IL 8受体的结合亲和力。接下来，我们使用[18F]脯氨酸合成[18F]IL-8。经离子交换层析后，可在短时间内得到放化纯度为91%的[18F]IL-8。

First, we synthesized [19F]-interleukin-8 (IL-8) which contained non-labelled fluoroproline instead of proline using the cell-free protein synthesis system. We confirmed that pure and abundant [19F]-IL-8 could be obtained. The binding affinity of [19F]IlL-8 to IL8 receptors was retained. Next, we synthesized [18F]IL-8 using [18F]proline. After ion-exchanged chromatography, we could obtained [18F]IL-8 which had 91% of radiochemical purity in a short time.