Coordinated epigenetic regulation of cell type-specific genes and chronic disease development in cardiomyocytes.

在这项研究中，我从几个发育阶段的分离心肌细胞群体中获得了几种细胞类型特异性DNA甲基化表观基因组数据，并推动了对成熟细胞中表观基因组的理解。我将心肌细胞的表观基因组与胚胎干细胞和肝脏的表观基因组进行了比较，并与心力衰竭的心肌细胞进行了比较。结果，在其基因体区域中具有显著DNA甲基化变化的基因的小亚群被包括在细胞类型特异性基因性状中。这些基因体区域在成熟细胞中积累了转录机器和组蛋白乙酰化酶，并瞬时积累了甲基胞嘧啶去甲基化的衍生物，这暗示了主动的去甲基化过程。我们的研究表明，基因体区域参与了活跃的DNA去甲基化过程，并表明基因体DNA低甲基化可能稳定细胞类型特异性基因转录。

In this study, I made several cell type-specific DNA methylation epigenome data of isolated cardiomyocyte populations from several developmental stages and pushed forward understanding of the epigenome in matured cells. I compared the cardiomyocyte epigenome with those of embryonic stem cells and liver, and also with the heart failure cardiac muscle cells. As the result, small subpopulation of genes with remarkable DNA methylation changes in their gene body regions were included in the cell type-specific gene trait. These gene body regions accumulated the transcription machinery and histone acetylation enzyme in the matured cells, and transiently accumulate the derivatives of methyl cytosine demethylation, which implicates the active demethylation process. Our study showed the involvement of the active DNA demethylation process in gene body regions and indicates the possibility that the gene body DNA hypomethylationmay stabilize the cell type-specific gene transcription.