Development of on-line Raman detection system for the assistance of protein analysis

辅助蛋白质分析的在线拉曼检测系统的开发

• 批准号: 24710267
• 负责人: ANDO Jun
• 金额: $ 2.83万
• 依托单位: Osaka University (2013)The Institute of Physical and Chemical Research (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24710267/
• 关键词: ラマン分光法; 薬剤標識タンパク質; 表面増強ラマン散乱; 質量分析法; 液体クロマトグラフィー; タンパク質; 小分子; 薬剤標識; 薬剤標的タンパク質

Functions of proteins working in a biological cell is regulated by the binding with various kinds of small molecules. Identification of specific proteins, which bind with the small molecules, and the information of their binding mechanism at molecular level, will largely contribute for the drug discovery and the study of protein function. However, complexity of biological samples often prevent to analyze them. In this research, the construction of the system was considered that can combine Raman spectroscopy with liquid chromatography. Since Raman spectroscopy has a variety of chemical information, it will contribute to analyze the samples, which are under fractionation with liquid chromatography.

在生物细胞中工作的蛋白质的功能是通过与各种小分子的结合来调节的。鉴定与小分子结合的特异性蛋白质，并在分子水平上了解其结合机制，将为药物发现和蛋白质功能研究做出重要贡献。然而，生物样本的复杂性往往妨碍对其进行分析。在本研究中，我们考虑建构一套能将联合收割机拉曼光谱与液相色谱结合的系统。由于拉曼光谱具有多种化学信息，它将有助于分析样品，这是在与液相色谱分离。

项目成果

Alkyne-tag Raman imaging for visualization of small molecules

用于小分子可视化的炔标签拉曼成像

• 发表时间: 2013
• 作者: Jun Ando;Hiroyuki Yamakoshi;Kosuke Dodo;Almar Palonpon;Satoshi Kawata;Mikiko Sodeoka
• 通讯作者: Mikiko Sodeoka

Alkyne-tag Raman imaging for visualizing small molecules in a living cell

用于可视化活细胞中小分子的炔标签拉曼成像

• 作者: Jun Ando;Hiroyuki Yamakoshi;Almar Palonpon;Kosuke Dodo;Satoshi Kawata;Katsumasa Fujita;Mikiko Sodeoka
• 通讯作者: Mikiko Sodeoka

Dynamic SERS imaging of cellular transport in 3D

细胞运输的 3D 动态 SERS 成像

• 发表时间: 2014
• 作者: Jun Ando;Kazuki Bando;Kai-Chih Huang;Nicholas Smith;Katsumasa Fujita;Satoshi Kawata
• 通讯作者: Satoshi Kawata

Raman microscopy for visualizing small molecules in live cells

拉曼显微镜用于观察活细胞中的小分子

• 发表时间: 2014
• 作者: Jun Ando;Kazuki Bando;Kai-Chih Huang;Nicholas Smith;Katsumasa Fujita;Satoshi Kawata;Jun Ando
• 通讯作者: Jun Ando

Dynamic SERS imaging with gold nanoparticles transported in a living cell

使用活细胞中运输的金纳米颗粒进行动态 SERS 成像

• 发表时间: 2013
• 作者: Katsumasa Fujita;Jun Ando;Nicholas Smith;Satoshi Kawata
• 通讯作者: Satoshi Kawata