Key mediators of the enhancer blocker function of Drosophila CTCF

果蝇 CTCF 增强子阻滞剂功能的关键介质

• 批准号: 5422448
• 负责人: Professor Dr. Rainer Renkawitz
• 金额: --
• 依托单位: Institut für Genetik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5422448?language=en
• 关键词: Key; mediators; enhancer; blocker; function

Functional eukaryotic gene units including all of their regulatory elements are insulated from each other to allow the action of regulatory elements on the appropriate gene. We and others have analyzed the DNA binding factor CTCF, which is the only protein known to mediate a directional blocking activity of enhancer elements in vertebrates. Despite many efforts from several laboratories, a mechanism mediating enhancer blocking has not been determined. In contrast, in Drosophila other factors have been descr ibed, mediating chromatin insulation and enhancer blocking. In this application, for the first time, we have the possibility to compare the properties of the unpublished Drosophila orthologue of CTCF with the function of vertebrate CTCF. We will use the advantages of both systems to identify CTCF domains and nucleosomal modifications involved in enhancer blocking in order to unravel the molecular mechanism of enhancer blocking.

功能性真核基因单元（包括其所有调节元件）彼此绝缘，以允许调节元件对适当的基因起作用。我们和其他人已经分析了DNA结合因子CTCF，这是唯一已知的介导脊椎动物中增强子元件的定向阻断活性的蛋白质。尽管几个实验室进行了许多努力，但尚未确定介导增强子阻断的机制。相反，在果蝇中，其他因子已被描述，介导染色质绝缘和增强子阻断。在本申请中，我们第一次有可能将未发表的果蝇CTCF直向同源物的性质与脊椎动物CTCF的功能进行比较。我们将利用这两个系统的优势，以确定CTCF结构域和核小体修饰参与增强子阻断，以解开增强子阻断的分子机制。