Thyroid Hormone Receptor Corepressors: Requirements and Selectivity for Histone Modifications Cell Culture

甲状腺激素受体辅阻遏物：组蛋白修饰的要求和选择性细胞培养

• 批准号: 5422406
• 负责人: Professor Dr. Aria Baniahmad
• 金额: --
• 依托单位: Institut für Humangenetik
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5422406?language=en
• 关键词: Thyroid; Hormone; Receptor; Corepressors; Requirements

Thyroid hormone (T3) is an important regulator of neuronal differentiation and maturation. The receptor for thyroid hormone (TR) is a ligand regulated transcription factor mediating both gene repression and activation. A number of different corepressors have been described to interact with TR. Corepressors bind either in a hormone-sensitive, hormone-independent or hormone-dependent manner to TR. However, it is not understood which biological functions individual corepressors play in the context of TR-mediat ed gene repression and activation as well as for TR-mediated neuronal differentiation and maturation. This project analyses the selectivity and specificity of corepressors in the context of chromatin through histone modifications (acetylation, methylation, phosphorylation and ubiquitinylation) of TR target genes as well as the impact of corepressors on neuronal differentiation and maturation.

甲状腺激素（T3）是神经元分化和成熟的重要调节因子。甲状腺激素受体（TR）是一种配体调控的转录因子，介导基因抑制和激活。已经描述了许多不同的辅阻遏物与TR相互作用。辅阻遏物以酶敏感、酶非依赖或酶依赖的方式与TR结合。然而，目前还不清楚在TR介导的艾德基因抑制和激活以及TR介导的神经元分化和成熟的背景下，单个辅阻遏物发挥哪些生物学功能。该项目通过TR靶基因的组蛋白修饰（乙酰化、甲基化、磷酸化和泛素化）分析辅阻遏物在染色质背景下的选择性和特异性，以及辅阻遏物对神经元分化和成熟的影响。