乾燥・塩ストレス応答と生育を制御するサイトカイニンシグナル因子の機能解析

1.耐旱ahp和arr高阶突变体的鉴定。根据正常条件下的生长速度，选择Ahp和arr多个突变体进行耐旱测定。结果表明，几个ahp和arr高阶突变体具有耐旱性，提示这些AHP和ARR基因可能参与了干旱响应的调控。2．低阶 ahp 和 arr 突变体的详细耐受性测定。检查ahp和arr低阶（包括单个突变体）的耐旱程度，以研究所选的AHP和ARR是否冗余地调节干旱响应。结果表明，一些AHP和ARR基因似乎对干旱反应具有冗余调节作用，很少有基因能够单独发挥作用，因为这些基因的个别功能丧失会导致对干旱的敏感性。3．鉴定调节干旱响应和/或生长的 AHP 和 ARR 基因。鉴定出单突变体或双突变体中的几个 AHP 和 ARR 基因调节干旱反应但不调节植物生长。然而，它们的高阶突变影响了植物的干旱反应和生长。结果表明，干旱响应可能受到单一 AHP 或 ARR 的影响；然而，生长是由两个以上的 AHP 或两个 ARR 基因控制的。

1. Identification of drought-tolerant ahp and arr high-order mutants. Ahp and arr multiple mutants were selected for drought tolerance assay based on their growth rate under normal conditions. Results showed that several ahp and arr high-order mutants were drought-tolerant, suggesting that these AHP and ARR genes may be involved in the regulation of drought response.2. Detailed tolerance assay of lower-order ahp and arr mutants. Examined degree of drought tolerance of ahp and arr lower-order, including single mutants, to study whether the selected AHPs and ARRs redundantly regulate drought response. Results showed that several AHP and ARR genes appear to redundantly regulate drought response, and few of them could act alone, as individual loss-of-function of these genes resulted in sensitivity to drought.3. Identification of AHP and ARR genes that regulate drought response and/or growth. Several AHP and ARR genes in single or double mutants that regulate drought response but not plant growth were identified. However, their higher-order mutations affected both plant drought response and growth. Results suggest that drought response can be affected by a single AHP or ARR; however, growth is controlled by more than two AHP or two ARR genes.