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乾燥・塩ストレス応答と生育を制御するサイトカイニンシグナル因子の機能解析

控制干旱/盐胁迫反应和生长的细胞分裂素信号因子的功能分析

基本信息

批准号：
25440149
负责人：
西山りゑ
金额：
$ 3.41万
依托单位：
Institute of Physical and Chemical Research
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2013
资助国家：
日本
起止时间：
2013-04-01 至 2016-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-25440149/
关键词：
environmental response 環境応答

项目摘要

1. Identification of drought-tolerant ahp and arr high-order mutants. Ahp and arr multiple mutants were selected for drought tolerance assay based on their growth rate under normal conditions. Results showed that several ahp and arr high-order mutants were drought-tolerant, suggesting that these AHP and ARR genes may be involved in the regulation of drought response.2. Detailed tolerance assay of lower-order ahp and arr mutants. Examined degree of drought tolerance of ahp and arr lower-order, including single mutants, to study whether the selected AHPs and ARRs redundantly regulate drought response. Results showed that several AHP and ARR genes appear to redundantly regulate drought response, and few of them could act alone, as individual loss-of-function of these genes resulted in sensitivity to drought.3. Identification of AHP and ARR genes that regulate drought response and/or growth. Several AHP and ARR genes in single or double mutants that regulate drought response but not plant growth were identified. However, their higher-order mutations affected both plant drought response and growth. Results suggest that drought response can be affected by a single AHP or ARR; however, growth is controlled by more than two AHP or two ARR genes.

1.抗旱性AHP和ARR高位突变体的鉴定根据AHP和ARR多个突变体在正常生长条件下的生长速率，筛选出两个突变体进行抗旱性鉴定。结果表明，几个AHP和ARR高位突变体都具有耐旱性，提示这些AHP和ARR基因可能参与了干旱反应的调控。低位ahp和arr突变体的详细耐受性分析。检测AHP和ARR的耐旱性，包括单个突变体，以研究所选择的AHP和ARRS是否冗余地调控干旱反应。结果表明，几个AHP和ARR基因似乎冗余地调控干旱反应，其中很少有单独作用，因为这些基因的个体功能丧失导致了对干旱的敏感性。鉴定调控干旱反应和/或生长的AHP和ARR基因。在单个或双个突变体中鉴定了几个调控干旱反应但不调控植物生长的AHP和ARR基因。然而，它们的高阶突变既影响植物的干旱反应，也影响植物的生长。结果表明，干旱反应可能受到单一的AHP或ARR基因的影响；然而，生长受到两个以上的AHP或ARR基因的控制。