乾燥・塩ストレス応答と生育を制御するサイトカイニンシグナル因子の機能解析

1.耐旱ahp和cba高阶突变体的鉴定。根据正常条件下的生长速率，选择Ahp和cnc复合突变体进行耐旱性测定。结果表明，多个ahp和ARR高阶突变体具有耐旱性，提示这些AHP和ARR基因可能参与了干旱反应的调控.低阶ahp和pcr突变体的详细耐受性测定。研究了ahp和arr低阶的耐旱程度，包括单突变体，以研究所选的ahp和arr是否冗余地调节干旱反应。结果表明，几个AHP和ARR基因似乎冗余地调节干旱反应，其中很少能单独发挥作用，因为这些基因的个体功能丧失导致了对干旱的敏感性.鉴定调节干旱反应和/或生长的AHP和ARR基因。几个AHP和ARR基因的单或双突变体，调节干旱反应，但不植物生长被确定。然而，它们的高阶突变影响植物干旱反应和生长。结果表明，干旱反应可以由一个单一的AHP或ARR的影响，但是，增长是由两个以上的AHP或两个ARR基因控制。

1. Identification of drought-tolerant ahp and arr high-order mutants. Ahp and arr multiple mutants were selected for drought tolerance assay based on their growth rate under normal conditions. Results showed that several ahp and arr high-order mutants were drought-tolerant, suggesting that these AHP and ARR genes may be involved in the regulation of drought response.2. Detailed tolerance assay of lower-order ahp and arr mutants. Examined degree of drought tolerance of ahp and arr lower-order, including single mutants, to study whether the selected AHPs and ARRs redundantly regulate drought response. Results showed that several AHP and ARR genes appear to redundantly regulate drought response, and few of them could act alone, as individual loss-of-function of these genes resulted in sensitivity to drought.3. Identification of AHP and ARR genes that regulate drought response and/or growth. Several AHP and ARR genes in single or double mutants that regulate drought response but not plant growth were identified. However, their higher-order mutations affected both plant drought response and growth. Results suggest that drought response can be affected by a single AHP or ARR; however, growth is controlled by more than two AHP or two ARR genes.