Nano- and microfluidics using optical tweezers with fast single particle tracking

使用具有快速单粒子跟踪功能的光镊的纳米和微流体

• 批准号: 5425176
• 负责人: Professor Dr. Friedrich Kremer
• 金额: --
• 依托单位: Peter-Debye-Institut für Physik der weichen Materie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5425176?language=en
• 关键词: Nano; microfluidics; using; optical; tweezers

Optical tweezers with fast single particle tracking are microscopic rheometric tools with nanometer resolution in space and subpico -newton resolution in force. The proposed project has two intentions to contribute to basic questions in colloid- and polymer-research and to address technological problems of micro- and nanofluidics. In detail the following experiments are planned: 1.) Measurements of the force-distance dependence between two isolated single colloids of which one is hold by a micro-pipette and the other by optical tweezers. The separation between the colloids can be varied in nanometer steps. 2.) Measurement of the force-distance-dependence between a single colloid and a wall in the steady state and in fluid flow for coated and uncoated surfaces. 3.) Measurement of depletion forces between single colloids in the steady state and in flow for polymer solutions of varying concentration and for polymers of different topology. 4.) A fluctuation analysis of the Brownian motion of a colloid in an optical trap enables one to deduce the local tensor of viscosity. By that inhomogeneous microscopic structures like microchannels or living biological systems can be explored. 5.) Measurement of the flow profile of homogeneous and hetereogeneous liquids in small confining geometries like plates with micrometer separation, microchannels, etc. with - and without surface modifications (e.g. hydrophobization).

单粒子快速跟踪光镊是一种空间分辨率为纳米级、力分辨率为亚皮牛顿级的微观流变测量工具。拟议的项目有两个意图，有助于胶体和聚合物研究的基本问题，并解决微和纳米流体的技术问题。详细地，计划进行以下实验：1.）测量了两个孤立的单胶体之间的力-距离关系，其中一个由微移液器保持，另一个由光镊保持。胶体之间的分离可以以纳米级变化。2.）的情况。在稳态和流体流动中，测量单个胶体和壁之间的力-距离依赖性，用于涂覆和未涂覆表面。3.）第三章测量不同浓度和不同拓扑结构的聚合物溶液在稳态和流动状态下单个胶体之间的消耗力。4.）对胶体在光阱中的布朗运动进行涨落分析，可以导出胶体的局部粘滞张量。通过这种方法，可以探索不均匀的微观结构，如微通道或活的生物系统。5.）测量均匀和非均匀液体在小的限制几何形状中的流动轮廓，如带有微米分离的板、微通道等，具有和不具有表面改性（例如疏水化）。