Identification and characterisation of virulence associated genes during oral infections with Candida albicans

白色念珠菌口腔感染期间毒力相关基因的鉴定和表征

• 批准号: 5426840
• 负责人: Professor Dr. Bernhard Hube
• 金额: --
• 依托单位: Leibniz-Institut für Naturstoff-Forschung und Infektionsbiologie e. V. Hans-Knöll-Institut
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5426840?language=en
• 关键词: Identification; characterisation; virulence; associated; genes

Oral infections with Candida albicans are very common diseases in immunocompromised patients. The aim of this project is to identify fungal factors which are associated with oral infection and to elucidate the relevance and role of these factors. C. albicans DNA microarrays will be used to identify genes that are up-regulated or exclusively expressed in an established in vitro model of oral Candida infections (RHE) and in patient samples. Using the RHE model, we will dissect the influence of several host components such as epithelial cells, saliva, antimicrobial peptides, leukocytes and pH on the fungal expression profile. The expression pattern of selected genes will be confirmed by qualitative expression analysis. Finally, we will analyse the relevance and role of selected infection-associated genes by the production of null mutant strains and a detailed analysis of the gene products. Null mutants will then be analysed in the RHE model in order to investigate the impact of the corresponding gene on pat hogenesis. In co-operation with other applicants of this priority programme, we will also analyse the host response during oral infections and compare our transcriptional profiling data with data from vaginal infections. This information will not only help to understand how C. albicans is able to cause disease, but will also identify factors of C. albicans which if suppressed, may enable us to prevent oral infections and will point to host factors crucial for prevention of oral candidosis.

口腔念珠菌感染是免疫功能低下患者的常见疾病。本项目的目的是确定与口腔感染相关的真菌因素，并阐明这些因素的相关性和作用。C.白色念珠菌DNA微阵列将用于鉴定在已建立的口腔念珠菌感染（RHE）体外模型和患者样本中上调或专门表达的基因。使用RHE模型，我们将剖析几种宿主成分，如上皮细胞，唾液，抗菌肽，白细胞和pH值对真菌表达谱的影响。将通过定性表达分析确认选定基因的表达模式。最后，我们将通过无效突变株的产生和基因产物的详细分析来分析选定的感染相关基因的相关性和作用。然后在RHE模型中分析突变体，以研究相应基因对基因发生的影响。在与该优先计划的其他申请人的合作中，我们还将分析口腔感染期间的宿主反应，并将我们的转录谱数据与阴道感染的数据进行比较。这些信息不仅有助于理解C。白念珠菌是能够致病的，但也会鉴定出念珠菌的因子。白色念珠菌，如果抑制，可能使我们能够预防口腔感染，并指出宿主因素对预防口腔念珠菌病至关重要。