Characterisation of a novel type of promoter controlling expression of virulence genes in Neisseria.

控制奈瑟菌毒力基因表达的新型启动子的表征。

• 批准号: nhmrc : 194313
• 负责人: Prof John Davies
• 金额: $ 13.39万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2002
• 资助国家: 澳大利亚
• 起止时间: 2002-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/characterisation-novel-type-genes-neisseria/96308
• 关键词: Characterisation; novel; type; promoter; controlling

This project will investigate how two different types of bacteria control genes that are involved in determining their disease-causing ability. The expression of many bacterial genes is controlled by a sophisticated battery of regulatory systems that respond to individual, very specific, environmental signals. Such regulatory systems are capable of exerting very precise control over the level of gene expression, in response to the concentration of specific molecules in the immediate environment. However, there is evidence to suggest that many important disease-causing bacteria are much less reliant on specific regulatory systems. Instead, these bacteria rely more heavily what have been termed global systems for the regulation of gene expression. Such systems typically respond to less specific signals, such as the growth rate of the bacterial cell, but nevertheless appear capable of very precise control. We have evidence for a previously uncharacterised type of global control system that appears to be widespread amongst bacteria. It is likely that many virulence genes in a variety of disease-causing bacteria will prove to be controlled by similar means. Therefore this project will not only provide an insight into how expression of these particular virulence determinants is regulated, but will yield data that may help in our understanding of precise global regulatory processes in other bacterial species of medical importance.

该项目将研究两种不同类型的细菌如何控制决定其致病能力的基因。许多细菌基因的表达是由一系列复杂的调控系统控制的，这些系统对个体的、非常具体的环境信号做出反应。这种调节系统能够对基因表达水平施加非常精确的控制，以响应直接环境中特定分子的浓度。然而，有证据表明，许多重要的致病细菌对特定调控系统的依赖要小得多。相反，这些细菌更多地依赖于被称为基因表达调节的全局系统。这种系统通常对不太具体的信号做出反应，比如细菌细胞的生长速度，但似乎能够非常精确地控制。我们有证据表明，一种以前未被描述的全球控制系统似乎在细菌中广泛存在。很可能，各种致病细菌中的许多毒力基因将被证明是由类似的方法控制的。因此，该项目不仅将提供对这些特定毒力决定因素的表达如何被调控的见解，而且将产生可能有助于我们理解其他具有医学重要性的细菌物种的精确全球调控过程的数据。