Effects of Verticillium factors on cell biology and development of Arabidopsis thaliana and Brassica napus roots

植物的细胞骨架具有结构功能，并提供细胞内的运输和信号网络。真菌感染后，肌动蛋白细胞骨架发生深刻的重排。细胞骨架的变化可能是植物病原体防御系统的一部分。本研究旨在探讨大丽花黄萎病菌和长孢黄萎病菌感染对拟南芥和甘蓝型油菜肌动蛋白细胞骨架的影响及其对发育的影响。含有假定毒素的黄萎病菌提取物的活性将在表达纤维蛋白-绿色荧光蛋白的转基因植物中进行测试，以便在体内实时观察肌动蛋白细胞骨架。肌动蛋白细胞骨架的结构和动力学变化影响细胞内分泌和胞外分泌，并可能导致生长素载体定位的变化。因此，根系的生长发育可能受到干扰。这些过程将在黄萎病提取物处理过的植物中使用亚细胞标记物进行研究。

The cytoskeleton of plants has structural function and provides a network of transport and signalling within the cell. Upon fungal infection a profound rearrangement of the actin cytoskeleton takes place. Changes in the cytoskeleton may be part of the pathogen defence systems of plants. The aim of this proposal is to investigate the effect of Verticillium dahliae and Verticillium longisporum infection on the actin cytoskeleton of Arabidopsis thaliana and Brassica napus along with the developmental consequences of such effects. The activity of Verticillium extracts containing putative toxins will be tested in transgenic plants expressing fimbrin-GFP to allow visualisation of the actin cytoskeleton in vivo and real time. Changes in structure and dynamics of the actin cytoskeleton affect endo- and exocytosis and possibly result in changes in the localisation of auxin carriers. As a consequence root growth and development might be disturbed. These processes will be studied using subcellular markers in plants treated with Verticillium extracts.