Effects of Verticillium factors on cell biology and development of Arabidopsis thaliana and Brassica napus roots

植物的细胞骨架具有结构功能，并在细胞内提供运输和信号网络。真菌感染后，肌动蛋白细胞骨架发生深刻的重排。细胞骨架的变化可能是植物病原体防御系统的一部分。本研究的目的是研究大丽轮枝菌和长孢轮枝菌感染对拟南芥和甘蓝型油菜肌动蛋白细胞骨架的影响沿着这种影响的发育后果。将在表达纤蛋白-GFP的转基因植物中测试含有推定毒素的轮枝菌属提取物的活性，以允许在体内和真实的时间观察肌动蛋白细胞骨架。肌动蛋白细胞骨架的结构和动力学的变化影响内分泌和胞吐作用，并可能导致生长素载体定位的变化。因此，根的生长和发育可能受到干扰。这些过程将在用轮枝菌提取物处理的植物中使用亚细胞标记进行研究。

The cytoskeleton of plants has structural function and provides a network of transport and signalling within the cell. Upon fungal infection a profound rearrangement of the actin cytoskeleton takes place. Changes in the cytoskeleton may be part of the pathogen defence systems of plants. The aim of this proposal is to investigate the effect of Verticillium dahliae and Verticillium longisporum infection on the actin cytoskeleton of Arabidopsis thaliana and Brassica napus along with the developmental consequences of such effects. The activity of Verticillium extracts containing putative toxins will be tested in transgenic plants expressing fimbrin-GFP to allow visualisation of the actin cytoskeleton in vivo and real time. Changes in structure and dynamics of the actin cytoskeleton affect endo- and exocytosis and possibly result in changes in the localisation of auxin carriers. As a consequence root growth and development might be disturbed. These processes will be studied using subcellular markers in plants treated with Verticillium extracts.