Effects of Verticillium factors on cell biology and development of Arabidopsis thaliana and Brassica napus roots

黄萎病因子对拟南芥和甘蓝型油菜根细胞生物学和发育的影响

• 批准号: 54309785
• 负责人: Privatdozent Dr. Thomas Teichmann
• 金额: --
• 依托单位: Institute of Science and Technology Austria
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/54309785?language=en
• 关键词: Effects; Verticillium; factors; cell; biology

The cytoskeleton of plants has structural function and provides a network of transport and signalling within the cell. Upon fungal infection a profound rearrangement of the actin cytoskeleton takes place. Changes in the cytoskeleton may be part of the pathogen defence systems of plants. The aim of this proposal is to investigate the effect of Verticillium dahliae and Verticillium longisporum infection on the actin cytoskeleton of Arabidopsis thaliana and Brassica napus along with the developmental consequences of such effects. The activity of Verticillium extracts containing putative toxins will be tested in transgenic plants expressing fimbrin-GFP to allow visualisation of the actin cytoskeleton in vivo and real time. Changes in structure and dynamics of the actin cytoskeleton affect endo- and exocytosis and possibly result in changes in the localisation of auxin carriers. As a consequence root growth and development might be disturbed. These processes will be studied using subcellular markers in plants treated with Verticillium extracts.

植物的细胞骨架具有结构功能，并在细胞内提供运输和信号网络。一旦真菌感染，肌动蛋白细胞骨架就会发生深刻的重排。细胞骨架的变化可能是植物病原防御系统的一部分。本研究的目的是研究黄萎病和黄萎病对拟南芥和甘蓝型油菜肌动蛋白细胞骨架的影响以及这种影响的发育后果。含有假定毒素的黄萎菌提取物的活性将在表达fimbrin-GFP的转基因植物中进行测试，以便在体内和实时显示肌动蛋白细胞骨架。肌动蛋白细胞骨架的结构和动力学的变化会影响细胞内和胞外的吞噬作用，并可能导致生长素载体定位的改变。因此，根的生长和发育可能会受到干扰。这些过程将在用黄萎菌提取物处理的植物中使用亚细胞标记进行研究。