Einfluss von Schwermetallen (Zink) auf die Pathogenität von Salmonella enterica pv. Typhimurium

在我的研究中，我将调查除铁以外的金属在细菌毒力中的作用。有许多致病微生物需要金属来产生毒力，包括结核分枝杆菌、脑膜炎奈瑟菌和肠炎沙门氏菌。我将首先使用沙门氏菌作为模式生物，因为它很容易被基因操纵。肠炎沙门氏菌。鼠伤寒杆菌的宿主范围很广，例如，它会导致人类肠胃炎。在小鼠模型中肠链球菌pv。鼠伤寒菌可作为肠炎沙门氏菌引起的伤寒的模型。人类的伤寒。铁、锰等不同微量元素对肠链球菌毒力的影响。鼠伤寒的研究部分得到了很好的研究。开始了解锌在肠链球菌毒力中的作用。鼠伤寒，第一个目标是：1)表征锌转运体在感染和生存中的作用，2)开始鉴定和表征涉及毒力的Zn2+依赖性酶，以及3)表征ZupT（确定膜拓扑结构和表征ZupT介导的金属运输）。

In my research I will investigate the role of metals other than iron in bacterial virulence. There are many pathogenic microorganisms that require metals for virulence including Mycobacterium tuberculosis, Neisseria meningitis and Salmonella enterica. I will first employ Salmonella as a model organism because it is easily genetically manipulated. Salmonella enterica pv. Typhimurium has a broad host range and it is for example responsible for human gastroenteritis. In the mouse model S. enterica pv. Typhimurium can serve as a model for typhoid fever caused by S. enterica pv. Typhi in humans. The role of different trace elements, such as iron and manganese, on virulence of S. enterica pv. Typhimurium are partially well investigated. Starting to understand the role of zinc in virulence of S. enterica pv. Typhimurium, the frist goals are: i) characterization the role of zinc transporters in infection and survival, ii) starting identification and characterization of Zn2+-dependent enzymes involved in virulence, and iii) characterization of ZupT (determining membrane topology and characterization of ZupT mediated metal transport).