Elucidation of the binding of the C1 carrier, tetrahydromethanopterin, and its derivatives, to enzymes involved in the energy metabolism pathway of methanogenic archaea

阐明 C1 载体四氢甲烷蝶呤及其衍生物与产甲烷古菌能量代谢途径中涉及的酶的结合

• 批准号: 5434997
• 负责人: Privatdozent Dr. Ulrich Ermler
• 金额: --
• 依托单位: Abteilung Molekulare Membranbiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2008-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5434997?language=en
• 关键词: Elucidation; binding; C1; carrier; tetrahydromethanopterin

In methanogenic and sulfate-reducing archaea tetrahydromethanopterin (H4MPT) acts as a C1 carrier between formyl and methyl oxidation levels. It is a coenzyme in many enzymatic reactions and is structurally analogous to tetrahydrofolate (h4F) - the C1 carrier in most other organisms. This project aims to study the binding of tetrahydromethanopterin, and its derivatives, to enzymes involved in the energy metabolism pathway of methanogenic archaea. The major emphasis will be towards solving the structure of the enzyme-coenzyme complexis by X-ray crystallography. These structures are expected to give important insight into the binding sites of the coenzyme and thus, into the mechanism of many enzymatic reactions critical to methanogenesis. Three enzymes - the methenly-H4MPT cyclohydrolase, the formylmethanofuran:H4MPT formyltransferase and the methyl-H4MPT:coenzyme M methyltransferase - from the methanogenic pathway have been chosen for this study. Additionally, the project aims to develop a fluorimetric assay for coenzyme bindung to these enzymes. Detailed description of aims, methods, wirk plan and time schedule are given separately.

在产甲烷和硫酸盐还原古菌中，四氢甲蝶呤（H4 MPT）作为甲酰基和甲基氧化水平之间的C1载体。它是许多酶促反应中的辅酶，在结构上类似于四氢叶酸（h4 F）-大多数其他生物体中的C1载体。本项目旨在研究四氢甲烷蝶呤及其衍生物与产甲烷古菌能量代谢途径相关酶的结合。主要的重点将是通过X射线晶体学来解决酶-辅酶复合物的结构。这些结构预计将提供重要的洞察辅酶的结合位点，从而进入许多关键的甲烷生成酶促反应的机制。三种酶-甲烯基-H4 MPT环化水解酶，甲酰甲烷呋喃：H4 MPT甲酰基转移酶和甲基-H4 MPT：辅酶M甲基转移酶-从产甲烷途径已被选择用于本研究。此外，该项目的目的是开发一种荧光测定辅酶结合这些酶。分别对研究的目的、方法、工作计划和时间安排进行了详细的描述。