X-ray structure analysis on key enzymes involved in the anaerobic degradation of hydrocarbons

碳氢化合物厌氧降解关键酶的X射线结构分析

• 批准号: 71697849
• 负责人: Privatdozent Dr. Ulrich Ermler
• 金额: --
• 依托单位: Abteilung Molekulare Membranbiologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2009
• 资助国家: 德国
• 起止时间: 2008-12-31 至 2015-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/71697849?language=en
• 关键词: ray; structure; analysis; key; enzymes

Protein structure analysis represents an essential link between the ecophysiological and the chemical aspects of the SPP1319 program. According to the concept of the SPP our group is supplied with purified proteins from biochemists of the program in order to initiate an X-ray structure analysis. In the first funding period we worked on benzyl-succinyl-CoA thiolase, acetophenone carboxylase, benzoyl-CoA reductase, benzoyl-CoA epoxidase and methyl-coenzyme M reductase. With the latter three enzymes (or enzyme fragments) a successful structure analysis was performed. In the new funding period we plan to work on succinyl-CoA(R) benzylsuccinate CoA transferase, benzyl-succinyl-CoA thiolase, acetone carboxylase, acetophenone carboxylase, benzoyl-CoA reductase, p-cresol methylhydrolase and methyl sulfide dependent methyltransferase.

蛋白质结构分析代表了SPP 1319计划的生态生理学和化学方面之间的重要联系。根据SPP的概念，我们的团队从该计划的生物化学家那里获得了纯化的蛋白质，以启动X射线结构分析。在第一个资助期，我们研究了苄基琥珀酰辅酶A硫解酶、苯乙酮羧化酶、苯甲酰辅酶A还原酶、苯甲酰辅酶A环氧酶和甲基辅酶M还原酶。用后三种酶（或酶片段）进行了成功的结构分析。在新的资助期内，我们计划研究琥珀酰辅酶A（R）苄基琥珀酸辅酶A转移酶、苄基琥珀酰辅酶A硫解酶、丙酮羧化酶、苯乙酮羧化酶、苯甲酰辅酶A还原酶、对甲酚甲基水解酶和甲基硫依赖性甲基转移酶。