Functional characterisation of the two-component signalling system of Arabidopsis

拟南芥二元信号系统的功能表征

• 批准号: 5436069
• 负责人: Professor Dr. Thomas Schmülling
• 金额: --
• 依托单位: Arbeitsgruppe Entwicklungsbiologie der Pflanze
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2004
• 资助国家: 德国
• 起止时间: 2003-12-31 至 2009-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5436069?language=en
• 关键词: Functional; characterisation; two; component; signalling

The objective of the proposal is to study specific aspects of the function of the two-component signalling system (TCS) in Arabidopsis. The TCS is, among the higher eukaryotes, unique to plants. Arabidopsis harbours eight hybrid sensor kinases (AHKs), five histidine phosphotransfer proteins (AHPs) and 22 genes encoding two different types of response regulators (type A and type B ARR genes). Most of the functions of members of the TCS, in particular in which signalling pathway they participate and how they realize information transfer via interactions with other proteins of Arabidopsis and through gene activation are unknown. We will contribute to an improved understanding of the TCS in two ways. We have cloned and expressed most of the TCS genes in order to identify proteinprotein interactions between proteins of the TCS and other proteins of the Arabidopsis proteome by means of twohybrid screens and affinity co-purification coupled with mass spectrometry. By genome-wide expression analysis we have identified a set of immediate early response genes to cytokinin, which signals through the TCS. These will serve, beside a general phenotypical analysis, as endogenous reporter genes to characterize single and multiple cytokinin receptor knockout mutants and lines expressing gain-of-function and dominant-negative TCS transcription factors in order to identify individual TCS pathways. The detailed analysis of these specific aspects of the TCS is complementary to programmes of other groups participating in AFGN or the 2010 project.

该提案的目的是研究拟南芥双组分信号系统（TCS）功能的具体方面。TCS是高等真核生物中植物所特有的。拟南芥含有8个杂交传感激酶（AHK）、5个组氨酸磷酸转移蛋白（AHPs）和22个编码两种不同类型的反应调节因子（A型和B型ARR基因）的基因。TCS成员的大部分功能，特别是它们参与的信号通路以及它们如何通过与拟南芥的其他蛋白质的相互作用和通过基因激活实现信息传递是未知的。我们将从两个方面促进对技合系统的了解。我们已经克隆和表达的TCS基因，以确定蛋白质之间的相互作用的TCS蛋白质和其他蛋白质的拟南芥蛋白质组通过双杂交筛选和亲和共纯化与质谱联用。通过全基因组表达分析，我们已经确定了一组立即早期反应基因的细胞分裂素，通过TCS的信号。除了一般表型分析之外，这些基因还将作为内源性报告基因来表征单个和多个细胞分裂素受体敲除突变体以及表达功能获得性和显性负性TCS转录因子的细胞系，以识别单个TCS途径。对技合战略这些具体方面的详细分析是对参与非洲渔业网或2010年项目的其他团体的方案的补充。