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Cytokinin as a regulator of chloroplast development and function

细胞分裂素作为叶绿体发育和功能的调节剂

基本信息

批准号：
202988077
负责人：
Professor Dr. Thomas Schmülling
金额：
--
依托单位：
Arbeitsgruppe Entwicklungsbiologie der Pflanze
依托单位国家：
德国
项目类别：
Research Grants
财政年份：
2011
资助国家：
德国
起止时间：
2010-12-31 至 2017-12-31
项目状态：
已结题

来源：
https://gepris.dfg.de/gepris/projekt/202988077?language=en
关键词：
Cytokinin regulator chloroplast development function

项目摘要

A role of the plant hormone cytokinin in regulating the development and activity of chloroplasts was described soon after its discovery more than fifty years ago. However, the molecular mechanisms underlying this activity remain elusive despite great progress in understanding the metabolism and cellular signalling of the hormone. This research proposal capitalizes on the availability of numerous novel cytokinin mutants and transgenic lines enabling new approaches to decipher cytokinin activities on plastids. In a first part, a detailed study of the consequences of cytokinin deficiency on plastid development and function will be performed in Arabidopsis thaliana. This will be accompanied by a study on the relevance of the plastids' own cytokinin metabolism using mutants of plastid-located cyto-kinin synthesizing enzymes, as well as enhanced plastid-specific cytokinin degradation. A significant activity of cytokinin on plastid development is a more rapid greening in light, which will form the basis to describe the role of cytokinin in plastid development, as well as to search for mutants of this activity. A hitherto unknown function of cytokinin, its protective function during light stress, will be studied in a third part of the project. Numerous stress-related parameters including production of reactive oxygen species, the scavenging system, lipid peroxidation and the D1 repair cycle will be analyzed in cytokinin mutants. A genetic approach is chosen to identify functionally relevant genes of this pathway.

植物激素细胞分裂素在50多年前被发现后不久就描述了它在调节叶绿体发育和活性中的作用。然而，尽管在了解激素的新陈代谢和细胞信号方面取得了很大进展，但这种活性背后的分子机制仍然难以捉摸。这项研究方案利用了许多新的细胞分裂素突变体和转基因株系的可用性，使得能够采用新的方法来破译叶绿体上的细胞分裂素活性。在第一部分中，将详细研究细胞分裂素缺乏对拟南芥叶绿体发育和功能的影响。与此同时，还将利用叶绿体定位的细胞分裂素合成酶的突变株来研究叶绿体自身的细胞分裂素代谢的相关性，以及增强叶绿体特有的细胞分裂素降解。细胞分裂素在叶绿体发育中的一个重要活性是在光照下变绿更快，这将成为描述细胞分裂素在叶绿体发育中的作用以及寻找这种活性的突变体的基础。该项目的第三部分将研究细胞分裂素迄今未知的功能，即它在轻度应激时的保护功能。在细胞分裂素突变体中，将分析许多与胁迫相关的参数，包括活性氧的产生、清除系统、脂质过氧化和D1修复周期。我们选择了一种遗传学的方法来鉴定该途径的功能相关基因。