食品機能性成分の効率的利用のためのナノ／マイクロキャリアの作製

今年，前一年建立的钙黄绿素渗漏机制同样影响了5-氟尿嘧啶（5-FU）和盐酸二甲双胍（MH）的包封率，分别是抗血小板和抗高血糖药物。对于吐温80稳定的囊泡悬浮液，5-FU和MH的包封率随着表面活性剂浓度的降低而增加，然后在较低的表面活性剂浓度下降低。这种趋势是由于双层去稳定/增溶和反胶束运输的综合作用。包封率的药物被发现是高于那些在文献中报道。但钙黄绿素的包封率明显高于药物。这种差异被解释为与它们不同的分子量和相对疏水性有关。同样发现囊泡悬浮液在4 °C下储存约一个月是稳定的。在储存期间，包封率略有下降，被认为是由于药物从囊泡的内部水相通过脂质双层到连续相的最小泄漏。这些研究表明，多重乳化和溶剂蒸发相结合的方法为制备具有可控尺寸和高包封率的亲水性生物活性分子的脂质囊泡提供了巨大的潜力，并将在制药，化妆品，食品和其他相关领域中找到工业应用。

In this year, the mechanisms of calcein leakage established in the previous year equally influenced the entrapment yields of 5-fluorouracil (5-FU) and metformin hydrochloride (MH), the antineoplatic and antihyperglycemic agents, respectively. For Tween 80 stabilized vesicle suspension, the entrapment yields of 5-FU and MH increased with decrease in the surfactant concentration, and then decreased at lower surfactant concentration. This trend was attributed to the combined effects of bilayer destabilization/solubilization and reversed-micellar transport. The entrapment yields of the drugs were found to be higher than those reported in the literature. However, the entrapment yield of calcein was remarkably higher than those of the drugs. The difference was explained in relation to their differing molecular weights and relative hydrophobicity. The vesicle suspension was equally found to be stable for about one month of storage at 4 °C. During storage, the entrapment yield decreased slightly and was considered to be due to minimal leakage of the drugs from the internal water phase of the vesicle to the continuous phase through the lipid bilayers. From these studies, the multiple emulsion and solvent evaporation integrated method offers great potentials for the formulation of lipid vesicles with controlled size and high entrapment yields of hydrophilic bioactive molecules, and would find industrial applications in pharmaceuticals, cosmetics, food, and other related fields.