食品機能性成分の効率的利用のためのナノ／マイクロキャリアの作製

在这一年中，前一年建立的钙黄蛋白渗漏机制同样影响了抗肿瘤药物5-氟尿嘧啶（5-FU）和抗高血糖药物盐酸二甲双胍（MH）的包封量。对于Tween 80稳定囊泡悬浮液，5-FU和MH的包封率随表面活性剂浓度的降低而增加，随表面活性剂浓度的降低而降低。这种趋势归因于双分子层不稳定/增溶和反胶束运输的综合作用。发现药物的包封率比文献报道的要高。钙黄蛋白的包封率明显高于其他药物。这种差异与它们不同的分子量和相对疏水性有关。在4°C的条件下，囊泡悬浮液同样可以稳定保存一个月左右。在储存过程中，包封率略有下降，这被认为是由于药物从囊泡内部水相通过脂质双分子层渗漏到连续相的情况很少。综上所述，多乳液与溶剂蒸发相结合的方法在制备尺寸可控、亲水生物活性分子包封率高的脂质囊泡方面具有很大的潜力，在医药、化妆品、食品等相关领域具有广泛的工业应用前景。

In this year, the mechanisms of calcein leakage established in the previous year equally influenced the entrapment yields of 5-fluorouracil (5-FU) and metformin hydrochloride (MH), the antineoplatic and antihyperglycemic agents, respectively. For Tween 80 stabilized vesicle suspension, the entrapment yields of 5-FU and MH increased with decrease in the surfactant concentration, and then decreased at lower surfactant concentration. This trend was attributed to the combined effects of bilayer destabilization/solubilization and reversed-micellar transport. The entrapment yields of the drugs were found to be higher than those reported in the literature. However, the entrapment yield of calcein was remarkably higher than those of the drugs. The difference was explained in relation to their differing molecular weights and relative hydrophobicity. The vesicle suspension was equally found to be stable for about one month of storage at 4 °C. During storage, the entrapment yield decreased slightly and was considered to be due to minimal leakage of the drugs from the internal water phase of the vesicle to the continuous phase through the lipid bilayers. From these studies, the multiple emulsion and solvent evaporation integrated method offers great potentials for the formulation of lipid vesicles with controlled size and high entrapment yields of hydrophilic bioactive molecules, and would find industrial applications in pharmaceuticals, cosmetics, food, and other related fields.