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Elucidation of signal transduction mechanism of tetrapyrrole-based retrograde signaling in plants

阐明植物中基于四吡咯的逆行信号转导机制

基本信息

批准号：
16F16090
负责人：
増田建
金额：
$ 1.41万
依托单位：
The University of Tokyo
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2016
资助国家：
日本
起止时间：
2016-07-27 至 2019-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16F16090/
关键词：
レトログレードシグナルヘム葉緑体核テトラピロール

项目摘要

In higher plants, chloroplast biogenesis involves the coordinated expression of the chloroplast and nuclear genomes, requiring information to be sent from the developing chloroplasts to the nucleus and vice versa. This is achieved through so-called retrograde and anterograde signaling pathways, respectively. Currently, the best model to explain the retrograde signaling is that a specific tetrapyrrole molecules generated by chloroplast as a signal to regulate the expression of nuclear genes required for chloroplast development. Although tetrapyrrole is recognized as an organelle-derived signal, the mechanism of transport from organelle to the nucleus and other organelles is still largely unknown. In this research project, signal transduction mechanism of tetrapyrrole-based retrograde signaling in Arabidopsis thaliana has been investigated.During this research, we have succeeded in identifying candidate proteins involved in tetrapyrrole-derived retrograde signaling. It is possible that the identified ABC transporter is involved in transfer of signaling tetrapyrrole metabolites through envelope membrane of plastids. Furthermore, we found that GUN1, which is the most important component of retrograde signaling, directly binds to tetrapyrrole. These insights will significantly proceed our knowledge about the mechanism of retrograde signaling.

在高等植物中，叶绿体生物发生涉及叶绿体和核基因组的协调表达，需要将信息从发育中的叶绿体发送到核，反之亦然。这是通过所谓的逆行和顺行信号通路，分别实现。目前，解释这种逆行信号传导的最佳模型是叶绿体产生的一种特定的四吡咯分子作为信号调节叶绿体发育所需的核基因的表达。虽然四吡咯被认为是一种细胞器来源的信号，但从细胞器到细胞核和其他细胞器的转运机制仍然很不清楚。本研究对拟南芥中四吡咯类化合物的逆行信号转导机制进行了研究，成功地鉴定了四吡咯类化合物逆行信号转导的候选蛋白。推测ABC转运蛋白可能参与了四吡咯代谢产物通过质体被膜的转运。此外，我们发现，GUN1，这是最重要的组成部分，逆行信号，直接结合到四吡咯。这些见解将大大推进我们对逆行信号机制的认识。