ニホンザルとオランウータンにおける内分泌動態に影響を与える要因について

影响日本猕猴和猩猩内分泌动态的因素

• 批准号: 16J00399
• 负责人: CICALISE TAKESHI RAFAELA
• 金额: $ 1.47万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-04-22 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J00399/
• 关键词: Macaca fuscata; Pongo pygmaeus; DHEAS; glucocorticoids; testosterone; enzyme immunoassay; stress; endocrinology; stress hormone

I developed a new Enzyme Immunoassay (EIA) to detect dehydroepiandrosterone-sulfate (DHEAS) levels in feces of Japanese macaques and orangutans collected in the previous year. I validated the assay by confirming that DHEAS levels increased after DHEA challenge in one male and one female Japanese macaques. Additional challenges using Adrenocorticotrophic hormone (ACTH) and human chorionic gonadotropin(hGC) in other monkeys revealed that the intra-muscular injection of ACTH and hCG did not stimulate the adrenal to secrete DHEAS in Japanese macaques. The study has been published at the International Journal of Primatology. I also published the results from the previous year in the journla Primates, related to the stress-reducing effect of hot springs in stress levels of Japanese macaques.Another part of this project included the validation of the EIA for measurement of DHEAS in feces of orangutans. I investigated hormonal changes (DHEAS and glucocorticoids) levels with: (a) stress response, (b) reproductive state, and (c) age in orangutans from captivity and from the wild. The results suggest that DHEAS rises in response to increase in GC levels. I also found that pregnant females had the highest levels of DHEAS, and that early lactating females had the highest levels of fGC. Finally, I found that DHEAS is lower in infants than in adults and adolescents, with a pre-pubertal increase on DHEAS levels. This phenomenon is called adrenarche, and it is present in humans, chimpanzees, and bonobos, but not in macaques and lower primates.

我开发了一种新的酶免疫测定法(EIA)来检测前一年收集的日本猕猴和猩猩粪便中的脱氢表雄酮-硫酸酯(DHEAS)水平。我确认了在DHEA攻击后，一只雄性和一只雌性日本猕猴的DHEAS水平增加，从而验证了这项测试。在其他猕猴身上使用促肾上腺皮质激素(ACTH)和人绒毛膜促性腺激素(HGC)的其他挑战表明，肌肉注射ACTH和hCG并没有刺激日本猕猴的肾上腺分泌DHEAS。这项研究已经发表在《国际灵长类动物学杂志》上。我还在灵长类动物期刊上发表了前一年的结果，与温泉对日本猕猴压力水平的缓解作用有关。该项目的另一部分包括验证用于测量猩猩粪便中DHEAS的EIA。我研究了激素水平的变化(DHEAS和糖皮质激素)：(A)应激反应，(B)繁殖状态，(C)圈养和野生猩猩的年龄。结果表明，DHEAS随着GC水平的升高而升高。我还发现，怀孕的女性DHEAS水平最高，哺乳期早期的女性FGC水平最高。最后，我发现婴儿的DHEAS水平低于成年人和青少年，青春期前DHEAS水平有所上升。这种现象被称为肾上腺素，它存在于人类、黑猩猩和黑猩猩身上，但不存在于猕猴和低等灵长类动物中。

项目成果

Social dominance and fecal steroids in castrated male Japanese macaques (Macaca fuscata)

阉割雄性日本猕猴（Macaca fuscata）的社会支配地位和粪便类固醇

• 发表时间: 2017
• 作者: Takeshita RSC;Huffman MA;Kinoshita K;Bercovitch FB
• 通讯作者: Bercovitch FB

Some like hot: Japanese macaques lower glucocorticoid levels by bathing in hot springs

有些人喜欢热：日本猕猴通过泡温泉来降低糖皮质激素水平

• 发表时间: 2017
• 作者: Takeshita RSC,Bercovitch F;Kinoshita K;Huffman MA
• 通讯作者: Huffman MA

The effect of castration and the environment on the relationship between dominance rank and fecal steroid concentrations in male Japanese macaques (Macaca fuscata)

去势和环境对雄性日本猕猴（Macaca fuscata）优势等级和粪便类固醇浓度关系的影响

• 发表时间: 2016
• 作者: Takeshita RSC;Huffman MA;Kinoshita K;Bercovitch FB
• 通讯作者: Bercovitch FB

The Effect of Hot Spring Bathing on Stress Levels in Japanese Macaques

温泉浴对日本猕猴压力水平的影响

• 发表时间: 2017
• 作者: Takeshita RSC;Bercovitch FB;Kinoshita K;Huffman MA
• 通讯作者: Huffman MA

Ten years of collaboration between France and Japan-Studies on reproduction in Japanese macaques

法国与日本合作十年——日本猕猴繁殖研究

• DOI: 10.4000/primatologie.2666
• 发表时间: 2016
• 期刊: Revue de Primatologie
• 作者: C Garcia;F Bercovtich;T Furuichi;MA Huffman;AJJ MacIntosh;L Rigaill;RSC Takeshita;K Shimizu
• 通讯作者: K Shimizu