Structural and Functional Links between Denitrifiers, Fermenters and Methanogens: Impact on Greenhouse Gases

反硝化器、发酵器和产甲烷菌之间的结构和功能联系：对温室气体的影响

• 批准号: 5445027
• 负责人: Professor Dr. Marcus A. Horn
• 金额: --
• 依托单位: Institut für Mikrobiologie (ifmb)
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2005
• 资助国家: 德国
• 起止时间: 2004-12-31 至 2011-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5445027?language=en
• 关键词: Structural; Functional; Links; between; Denitrifiers

Fens emit the greenhouse gases methane (CH4) and nitrous oxide (N20). In the fen evaluated in the previous funding period, pore-water methane concentrations and methanogenic potentials were lower after experimental drying and rewetting in 2006, whereas anaerobic CCVproduction potentials, surface-near nitrate concentrations, and nitrous oxide emissions were higher. Thus, the methanogenic food chain and denitrtfication were affected by drying and rewetting. The new objectives of the next funding period are to identify the effect of intensified drying and rewetting, as well as flooding on the structure and activity of (i) fermenters, (ii) methanogens, and (iii) denitrifiers. Denitrifier diversity will be identified by functional gene analysis (narG and nosZ) and cultivation methods. Novel organisms will be ecophysiologically characterized and taxonomically validated. Comparative mRNA and DNA based T-RFLP analyses of functional genes, qPCR, and 16S rRNA-based microarray analyses will be used to detect changes in community structure of active organisms. Michaelis-Menten-kinetics of fermenters, methanogens, and denitrifiers will be applied to identify community structure-related activity changes and thus elucidate structurefunction relationship of greenhouse-gas producing organisms in fens.

沼泽排放温室气体甲烷（CH 4）和一氧化二氮（N2 O）。在前一个供资期评估的沼泽，孔隙水甲烷浓度和产甲烷潜力在2006年实验干燥和再湿润后较低，而厌氧CCV生产潜力，表面附近的硝酸盐浓度和一氧化二氮排放量较高。因此，产甲烷食物链和反硝化作用受到干燥和再湿润的影响。下一个资助期的新目标是确定强化干燥和再润湿以及洪水对（i）发酵罐，（ii）产甲烷菌和（iii）发酵罐的结构和活性的影响。反硝化菌多样性将通过功能基因分析（narG和nosZ）和培养方法进行鉴定。新生物将进行生态生理学特征描述和分类学验证。将使用比较mRNA和基于DNA的功能基因T-RFLP分析、qPCR和基于16 S rRNA的微阵列分析来检测活性生物体群落结构的变化。利用发酵菌、产甲烷菌和厌氧微生物的米氏动力学研究了群落结构相关的活性变化，从而阐明了沼泽地温室气体产生菌的结构与功能的关系。