高圧噴射装置による細菌細胞溶解メカニズム解明と余剰汚泥削減への応用

高压注入装置阐明细菌细胞裂解机制及其在剩余污泥减量化中的应用

• 批准号: 16J08523
• 负责人: 謝 理
• 金额: $ 0.83万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2016
• 资助国家: 日本
• 起止时间: 2016-04-22 至 2018-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16J08523/
• 关键词: High pressure jet device; Sludge reduction; Cavitation; Anaerobic digestion; Methane production; Pretreatment; Cell disruption; Atomic force microscopy

In the PhD stage, I conducted a study to elucidate the mechanism of bacterial cell disruption in activated sludge treatment by using a high pressure jet device (HPJD) (2016 - 2017). The HPJD consists of a high pressure pump and a tubular pipe with two independent entrances. The lateral port of HPJD is connected to a high-pressure pump to transport sludge via a nozzle at a high lateral velocity while the top port receives sludge by aspiration. The dual supply of sludge from horizontal and vertical directions generates frictional shear, fine bubbles (as a result of cavitation), and collision. These multiple effects disrupt bacterial cells and allow the release of intercellular compounds from damaged cells resulting in sludge reduction. My study revealed that cavitation was the dominate effect for cell disruption (Published in Chemical Engineering Journal (IF=6.216) in 2017). After I got PhD degree in 2017, I focused on the study about application of HPJD as a pretreatment in sludge anaerobic digestion to enhance sludge reduction and methane production. The applicability of HPJD on sludge anaerobic digestion was already confirmed by lab scale digestion tests using excess activated sludge and seed sludge were collected from Nakagawa and Morigasaki Water Reclamation Center

在博士阶段，我进行了一项研究，通过使用高压射流装置（HPJD）阐明活性污泥处理中细菌细胞破裂的机制（2016 - 2017）。HPJD由一个高压泵和一个带有两个独立入口的管状管道组成。HPJD的侧端口连接到高压泵，以通过喷嘴以高横向速度输送污泥，而顶部端口通过抽吸接收污泥。来自水平和垂直方向的污泥的双重供应产生摩擦剪切、细气泡（作为空化的结果）和碰撞。这些多重作用破坏细菌细胞，并允许从受损细胞释放细胞间化合物，从而减少污泥。我的研究表明，空化是细胞破裂的主要影响（2017年发表在Chemical Engineering Journal（IF=6.216））。2017年获得博士学位后，我专注于研究HPJD作为污泥厌氧消化的预处理，以提高污泥减量和甲烷产量。实验室规模的污泥厌氧消化试验已经证实了HPJD的适用性，剩余活性污泥和种子污泥收集自中川和森崎水再生中心

项目成果

Elucidating a Dominant Effect of High-Pressure Jet Device on Bacterial Cell Disruption and Release of Intracellular Substances.

阐明高压喷射装置对细菌细胞破裂和细胞内物质释放的主要影响。

• 发表时间: 2016
• 作者: L. Xie;Q. Bao;A. Terada;M. Hosomi;Li Xie
• 通讯作者: Li Xie

Single-cell analysis of the disruption of bacteria with a high-pressure jet device: An application of atomic force microscopy

• DOI: 10.1016/j.cej.2016.07.112
• 发表时间: 2016-12
• 期刊: Chemical Engineering Journal
• 影响因子: 15.1
• 作者: Li Xie;Qian Bao;A. Terada;M. Hosomi

Disentangling the multiple effects of a novel high pressure jet device upon bacterial cell disruption

• DOI: 10.1016/j.cej.2017.04.067
• 发表时间: 2017-09
• 期刊: Chemical Engineering Journal
• 影响因子: 15.1
• 作者: Li Xie;A. Terada;M. Hosomi