土壌適応因子としてのダイズ根粒菌種の脱窒システム

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In the present research, co-culture experiments showed that B. japonicum is less competitive than B. diazoefficiens under low oxigen contidions. Also the heam staining of NapC and immunoblotting analysis of NapA indicated lower levels of Nap in B. japonicum than in B. diazoefficiens under low-oxygen conditions, in the presence of nitrate, as a possible reason for the disadvantage of B. japonicum. Also, the lower production of FixP and FixO protein (part of cbb3-type high-affinity cytochrome oxidase) may be related to the inability of B. japonicum to produce Nap in sufficient levels. These results support my previous observation that nitrate reduction capacity may be an important factor driving the distribution of bradyrhizobia in response to soil oxygen conditions.