A New Salting-out Phenomenon of Water-soluble Polymer at Solid-liquid Interface

水溶性聚合物固液界面盐析的新现象

• 批准号: 22J14976
• 负责人: GONG HAO
• 金额: $ 0.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-22 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22J14976/
• 关键词: LLPS; Separation; Solid-liquid Interface; DNA; PEGylation; Rapid; Salt

The research project on new salting-out phenomena of water-soluble polymers at the solid-liquid interface is currently in progress and is on track to meet the established goals and objectives. Here, I discovered that PEGs with the same molecular weight but distinct termini can be instantly separated when an aqueous solution of their mixture with a very high salt-to-PEG weight ratio (~10^6) is drop-cast on a glass plate. A mixture of four polymers can rapidly be separated. Even when their termini are structurally analogous to each other with only a two carbon-atom and hydrogen-atom difference, they can be rapidly separated. I have completed the important stages of the project, including literature review, research design, and data collection. The data collected has been analyzed using appropriate scientific means, and the findings have been interpreted.Currently, we are working on resubmitting the research manuscript to high-impact journal, Nature, which will include a detailed analysis of the findings, conclusions, and recommendations for future research. Extending work on this finding is also pushing forward from synthetic polymers to biological macromolecules such as DNAs, RNAs, and peptides. Next, I will prepare to present our findings at upcoming conferences and submit more articles to relevant journals for more publications.As for the established timeline and budget, the research project is also proceeding according to the plan, with no significant issues or delays. I remain committed to ensuring the project's success and achieving its intended outcomes.

关于水溶性聚合物在固液界面的新盐析现象的研究项目目前正在进行中，并正朝着既定的目标和目标迈进。在这里，我发现具有相同分子量但末端不同的peg可以立即分离，当盐与peg的重量比非常高（~10^6）的混合物的水溶液落在玻璃板上时。四种聚合物的混合物可以迅速分离。即使它们的末端在结构上彼此相似，只有两个碳原子和两个氢原子的区别，它们也能被迅速分离。我已经完成了项目的重要阶段，包括文献综述、研究设计、数据收集。使用适当的科学手段对收集到的数据进行了分析，并对研究结果进行了解释。目前，我们正在努力将研究手稿重新提交给高影响力期刊《自然》，其中将包括对研究结果、结论和未来研究建议的详细分析。对这一发现的扩展工作也从合成聚合物推进到生物大分子，如dna、rna和肽。接下来，我将准备在即将召开的会议上展示我们的发现，并向相关期刊提交更多的文章，以便更多地发表。在既定的时间和预算方面，研究项目也在按计划进行，没有出现重大问题和延误。我仍然致力于确保项目的成功和实现其预期的结果。