A New Salting-out Phenomenon of Water-soluble Polymer at Solid-liquid Interface

水溶性聚合物固液界面盐析的新现象

• 批准号: 22J14976
• 负责人: GONG HAO
• 金额: $ 0.58万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-22 至 2023-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22J14976/
• 关键词: LLPS; Separation; Solid-liquid Interface; DNA; PEGylation; Rapid; Salt

The research project on new salting-out phenomena of water-soluble polymers at the solid-liquid interface is currently in progress and is on track to meet the established goals and objectives. Here, I discovered that PEGs with the same molecular weight but distinct termini can be instantly separated when an aqueous solution of their mixture with a very high salt-to-PEG weight ratio (~10^6) is drop-cast on a glass plate. A mixture of four polymers can rapidly be separated. Even when their termini are structurally analogous to each other with only a two carbon-atom and hydrogen-atom difference, they can be rapidly separated. I have completed the important stages of the project, including literature review, research design, and data collection. The data collected has been analyzed using appropriate scientific means, and the findings have been interpreted.Currently, we are working on resubmitting the research manuscript to high-impact journal, Nature, which will include a detailed analysis of the findings, conclusions, and recommendations for future research. Extending work on this finding is also pushing forward from synthetic polymers to biological macromolecules such as DNAs, RNAs, and peptides. Next, I will prepare to present our findings at upcoming conferences and submit more articles to relevant journals for more publications.As for the established timeline and budget, the research project is also proceeding according to the plan, with no significant issues or delays. I remain committed to ensuring the project's success and achieving its intended outcomes.

关于水溶性聚合物在固-液界面的新盐析现象的研究项目目前正在进行中，有望实现既定的目标和目的。在这里，我发现具有相同分子质量但不同末端的钉子可以立即分离，当它们的混合物的水溶液具有非常高的盐与聚乙二醇重比(约10^6)时，将其滴注在玻璃板上。四种聚合物的混合物可以快速分离。即使它们的末端在结构上彼此相似，只有两个碳原子和两个氢原子的差异，它们也可以迅速分离。我已经完成了项目的重要阶段，包括文献综述、研究设计和数据收集。收集的数据已经用适当的科学手段进行了分析，并对结果进行了解释。目前，我们正在努力将研究手稿重新提交给影响力较大的杂志《自然》，其中将包括对发现、结论和对未来研究的建议的详细分析。这一发现的扩展工作也在推动从合成聚合物到生物大分子，如DNA、RNA和多肽。接下来，我将准备在即将到来的会议上发表我们的研究成果，并向相关期刊提交更多文章以供更多文章发表。至于既定的时间表和预算，研究项目也在按计划进行，没有重大问题或延误。我仍然致力于确保项目的成功和实现预期的结果。