木化の力学的意義：植物細胞壁モデルの人工木化の解析

木质化的力学意义：植物细胞壁模型人工木质化分析

• 批准号: 21K19144
• 负责人: 阿部 賢太郎
• 金额: $ 3.91万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Research (Exploratory)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-07-09 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K19144/
• 关键词: Cellulose; Nanofiber; Microfibril; Lignification; Growth stress; 木化; セルロースナノファイバー; 成長応力; 樹木

今年度は木材から単離したセルロースミクロフィブリル（CMF）から，CMFゲルを作製し，その内部に人工リグニンを十分に堆積させるための詳細な実験条件を検討した．CMFゲルは木材本来のミクロフィブリルの形状および結晶性を有していることは確認済みである．人工リグニンは基質（今回はコニフェリルアルコール），酵素（ホースラデッシュペルオキシダーゼ）および過酸化水素水を混ぜることで簡単に作製可能である．しかし，ゲル外部で人工リグニンを反応させると，重合と凝集によって巨大なリグニン粒子が形成されるため，緻密なネットワーク構造を持つゲルの内部にその粒子が侵入することができなくなる．そこで，本研究ではエタノールを用いることで，リグニンの重合と凝集（とくに凝集）を阻害した．上記の原料をエタノール水溶液に溶解させ，ゲルを十分に浸漬させた．この時点では液は透明である．その後，低圧下でエタノールを優先的に蒸発させることで，ゲル内部でリグニンの堆積が可能となった．この工程を繰り返すことでゲル内部に徐々にリグニンを堆積させることができ，その様子が電子顕微鏡で観察された．ゲルを使用することの利点は簡便に力学試験に供することができることであり，ゲル中のリグニン量と引張特性の関係が明らかになった．また，リグニン量の増加とともに，ゲルの含水率が低下することも確認されている．同時に，本実験によって樹木の成長応力発生要因の一つとされるリグニン膨潤仮説についても重要な示唆が得られた，

This year, the wood is separated from each other and the CMF must be prepared. The CMF must be prepared according to the detailed conditions of the wood. The CMF must be prepared according to the shape and crystallinity of the wood. Artificial intelligence is the substrate (this time around), enzyme (this time around) and hyperacidified water. The outer part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the outer part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the outer part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the outer part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the outer part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner part of the body. The inner part of the body is artificially separated from the inner In this study, we found that the aggregation of the two groups was harmful. Note that the raw materials are dissolved in aqueous solution.この时点では液は透明である. After that, under low pressure, the priority of evaporation is to reduce the internal accumulation of impurities. This project is based on the observation of electron micromirrors. The relationship between the strength and tensile properties of the material is simple. The increase in water content was confirmed. At the same time, the growth and development of trees are mainly due to the growth and expansion of trees.