Elucidating transmission dynamics for soil-transmitted helminths based on environmental DNA analysis

基于环境 DNA 分析阐明土源性蠕虫的传播动力学

• 批准号: 21K12269
• 负责人: オオタケサトウ マルセロ
• 金额: $ 2.5万
• 依托单位: Dokkyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2021
• 资助国家: 日本
• 起止时间: 2021-04-01 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21K12269/
• 关键词: Ecohealth; One-health; environmental DNA; STH; qPCR; STHs; prophylaxis

Goal: Improvement of soil transmitted helminths-specific DNA detection method.The analyses of DNA sequences of the parasite species causing STH retrieved from the database allowed us to develop suitable probes for STH detection.We designed the environmental DNA detection assay for soil samples, using mitochondrial COX1 gene. The specific sets of primers and probes were designed within an expected short length of the amplified DNA (about 100 to 200 bp).Tests using DNA extracted from adult worms (target and non-target) showed the assays are suitable with a high specificity. However, it needs improvement on the sensitivity, for detection of small quantities of target DNA usually found eDNA.We are now working on a new technique based in multi-probe detection which increases the sensitivity of the assay without loss in specificity (as reported by our group in detection of Opisthorchis viverrini in eDNA from water samples).

目标：改进土壤传播的蠕虫特异性 DNA 检测方法。对从数据库中检索到的引起 STH 的寄生虫物种的 DNA 序列进行分析，使我们能够开发出适合 STH 检测的探针。我们使用线粒体 COX1 基因设计了土壤样品的环境 DNA 检测方法。特定的引物和探针组是在扩增 DNA 的预期短长度（约 100 至 200 bp）内设计的。使用从成虫（目标和非目标）提取的 DNA 进行的测试表明，该测定法具有高特异性。然而，它的灵敏度需要改进，用于检测通常在 eDNA 中发现的少量目标 DNA。我们现在正在研究一种基于多探针检测的新技术，该技术可以提高检测的灵敏度而不损失特异性（正如我们小组在检测水样 eDNA 中的 Opisthorchis viverrini 中所报告的那样）。

项目成果

Mahidol大学(タイ)

玛希隆大学（泰国）