In vitro Germ Cell Differentiation of Cord-Blood-derived Unrestricted Somatic Stem Cells (USSC)

人脐血来源的无限制体细胞(USSC)可以被重新编程为诱导的多能干细胞(IPS)。本项目通过不同的方法从USSC(IP-USSC)派生和表征iPS细胞。重新编程的脐带血细胞的分化潜力将通过与天然USSC进行比较来评估。该项目的重点包括从IP-USSC诱导中胚层/造血细胞和生殖细胞。此外，还将评估在USSC中诱导中胚层祖细胞的完全多能性。

Human cord-blood derived unrestricted somatic stem cells (USSC) can be reprogrammed to induced pluripotent stem (iPS) cells. This project derives and characterizes iPS cells from USSC (iP-USSC) by different means. The differentiation potential of reprogrammed cord blood cells will be assessed in comparison to native USSC. The focus of this project embraces mesodermal / hematopoietic and germ cell induction from iP-USSC. Besides reprogramming to full pluripotency induction of mesodermal progenitors in USSC will be evaluated.