Novel strategies to expand the scope of biological nanopores for sensing biomolecules

大部分预期的研究目标已经实现。特别是，设计的蛋白质已经在小范围内表达和纯化。大规模生产正在进行中。化学合成已经重复进行了。化学品的纯度正在进一步提高。UPLC-MS系统的建立被推迟，但它目前显著提高了化学品的纯度和特征。另一方面，AKTAgo FPLC系统的交付由于日元贬值和新冠疫情期间国际交付困难而大大推迟。然而，它已经建立起来，蛋白质的纯度正在大大提高。新型纳米孔的制备目前正在进行中。上述仪器的建立和台式核磁共振系统的建立将大大加快项目的进展。

Most of the expected research goals have been achieved. Particularly, designed proteins have been expressed and purified in small scales. Larger scale production is in progress. The chemical syntheses have been repeated. The purity of the chemicals is being further improved. The establishment of a UPLC-MS system is delayed but it is currently significantly improving the purity and characterisation of the chemicals. On the other hand, the delivery of the AKTAgo FPLC system was much delayed due to the devaluation of the Japanese Yen and difficulties in international delivery during the COVID pandemic. However, it has been set up and the purity of the proteins is being greatly improved. The preparation of novel nanopores are currently in progress. The establishments of the above-mentioned instruments together with a Benchtop NMR system will accelerate the progress of the project greatly.