Novel strategies to expand the scope of biological nanopores for sensing biomolecules

扩大生物纳米孔传感生物分子范围的新策略

• 批准号: 22K05330
• 负责人: Kong Lingbing
• 金额: $ 2.75万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2022
• 资助国家: 日本
• 起止时间: 2022-04-01 至 2027-03-31
• 项目状态: 未结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22K05330/
• 关键词: nanopore

Most of the expected research goals have been achieved. Particularly, designed proteins have been expressed and purified in small scales. Larger scale production is in progress. The chemical syntheses have been repeated. The purity of the chemicals is being further improved. The establishment of a UPLC-MS system is delayed but it is currently significantly improving the purity and characterisation of the chemicals. On the other hand, the delivery of the AKTAgo FPLC system was much delayed due to the devaluation of the Japanese Yen and difficulties in international delivery during the COVID pandemic. However, it has been set up and the purity of the proteins is being greatly improved. The preparation of novel nanopores are currently in progress. The establishments of the above-mentioned instruments together with a Benchtop NMR system will accelerate the progress of the project greatly.

大部分预期的研究目标已经实现。特别是，设计的蛋白质已经在小规模表达和纯化。大规模生产正在进行中。化学合成重复了一遍。化学品的纯度正在进一步提高。UPLC-MS系统的建立被推迟，但目前正在显着提高化学品的纯度和特性。另一方面，由于新冠疫情期间日圆贬值及国际交付困难，AKTAgo FPLC系统的交付大幅延迟。然而，它已经建立，蛋白质的纯度正在大大提高。新型纳米孔的制备目前正在进行中。上述仪器的建立和台式核磁共振系统的建立将大大加快项目的进度。

项目成果

Multidisciplinary studies of the effect of capsular polysaccharide transporter inhibitors on plant bacterial pathogens

荚膜多糖转运蛋白抑制剂对植物细菌病原体影响的多学科研究

• 发表时间: 2022
• 作者: KONG Lingbing