Nanopore sensors for multiplexed, ultra-fast gene detection

用于多重、超快速基因检测的纳米孔传感器

• 批准号: LP220200182
• 负责人: A/Prof Michelle Dunstone
• 金额: $ 28.7万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2023
• 资助国家: 澳大利亚
• 起止时间: 2023-07-01 至 2026-06-30
• 项目状态: 未结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP220200182
• 关键词: Nanopore; sensors; multiplexed; ultra; fast

The aim of this research is to develop the application of protein nanopores for multiplex identification of DNA samples for ultrafast gene detection. This is a type of barcoding of organism DNA that allows for rapid gene identification. This technology aims to address a significant need for rapid, on-the-spot identification of organisms. Applications include rapid identification of pathogenic bacteria in infections and identification of organisms in environmental sampling. Current methods are relative slow, require DNA amplification and specialised laboratories. This proposal aims to fine tune the properties of the large nanopore, polyC9, with respect to size and charge, as well as to identify and characterise novel large nanopores.

本研究的目的是开发蛋白质纳米孔在超快基因检测中用于DNA样品多重鉴定的应用。这是一种生物体DNA条形码，可以快速识别基因。该技术旨在满足快速、现场鉴定生物体的重大需求。应用包括快速鉴定感染中的致病菌和鉴定环境取样中的生物体。目前的方法相对较慢，需要DNA扩增和专门的实验室。 该提案旨在微调大纳米孔polyC9在尺寸和电荷方面的性质，以及识别和识别新的大纳米孔。